Pharmacognostic Evaluation and Antioxidant Activity of Urtica dioica L.

DOI: 10.4236/cm.2012.33021   PDF   HTML   XML   5,333 Downloads   10,705 Views   Citations

Abstract

Background: Urtica dioica L. is a common Himalayan species which produces allergenic substances causing oedema and inflammation in humans. It has become a source of folk medicine for the treatment of many diseases. The leaves and roots both are used internally as a blood purifier and diuretic and an infusion of the plant is used for nasal and menstrual haemorrhage, diabetes, rheumatism, eczema, anaemia, hair loss, as an expectorant and antidiarrhoeal. Present study includes pharmacognostic evaluation, antioxidant activity and HPTLC analysis of Urtica dioica L. Methods: Pharmacognostic evaluation of aerial part of U. dioica has been performed as per Indian pharmacopoeia. In-vitro antioxidant evaluation of U. dioica has been performed using DPPH free radical scavenging activity. Ferulic acid, a potential phenolic antioxidant present in this species, has been studied through HPTLC. Results: U. dioica hydro-alcoholic extract shows positive results for antioxidant activity with IC50 value of 88.33 ± 2.88 μg/ml. Standard ascorbic acid showed IC50 value of 2.8 ± 0.62 μg/ml. Ferulic acid was identified at Rf 0.61 ± 0.01 and quantified to 0.73% in this species through CAMAG HPTLC analysis. Conclusion: The pharmacognostical parameters reported can be considered as quality standards of U. dioica in herbal industry. Hydro-alcoholic extract of U. dioica showed positive in vitro antioxidant activity. Presence of phenolic compound suggests that antioxidant activity may be due to ferulic acid content.

Share and Cite:

V. Khare, P. Kushwaha, S. Verma, A. Gupta, S. Srivastava and A. Rawat, "Pharmacognostic Evaluation and Antioxidant Activity of Urtica dioica L.," Chinese Medicine, Vol. 3 No. 3, 2012, pp. 128-135. doi: 10.4236/cm.2012.33021.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] L. T. Ozen and H. Korkmaz, “Modulatory Effect of Urtica dioica,” Phytomedicine, Vol. 10, No. 5, 2003, pp. 405-415.
[2] H. Wetherilt, “Evaluation of Urtica Species as Potential Sources of Important Nutrients,” Developments in Food Science, Vol. 29, 1992, pp. 15-25.
[3] G. Granier, L. Bezanger-Beauquense and G. Debraux, “Ressources Medicinales de la Flore Francaise,” Vigot Freres, Paris, Vol. 2, 1961, pp. 962-964.
[4] A. Ziyyat, A. Legssyer, H. Mekhfi, A. Dassouli, M. Serhrouhni and W. Benjelloun, “Phytotherapy of Hypertension and Diabetes in Oriental Morocco,” Journal of Ethnopharmacology, Vol. 58, No. 1, 1997, pp. 45-54. doi:10.1016/S0378-8741(97)00077-9
[5] K. Reihemann, B. Behnke and K. Schulze-Osthoff, “Plant Extract from Stinging Nettle (Urtica dioica), an Antirheumatic Remedy, Inhibit the Pro-Inflammatory Transcription Factor,” FEBS Letters, Vol. 442, No. 1, 1999, pp. 89-94. doi:10.1016/S0014-5793(98)01622-6
[6] T. Krzeski, M. Kazon, A. Brokowski and J. Kuczera, “Combined Extracts Urtica dioica and Pygeum african in the Treatment of Hyperplasia: Double-Blind Comparison of Two Doses,” Clinical Therapeutics, Vol.6, No. 15, 1993, pp. 1011-1020.
[7] J. J. Lichius and C. Muth, “The Inhibiting Effect of Urtica dioica Root Extracts on Experimentally Induced Prostatic Hyperplasia in the Mouse,” Planta Medica, Vol. 63, No. 4, 1997, pp. 307-310. doi:10.1055/s-2006-957688
[8] A. Tahri, Y. Sabah, A. Legssyer, M. Aziz, H. Mekhfi, M. Bnnouham and A. Ziyyat, “Acute Diuteric, Natriuretic and Hypotensive Effects of a Continuous Perfusion of Aqueous Extract of Urtica dioica in the Rat,” Journal of Ethnopharmacology, Vol. 73, No. 1-2, 2000, pp. 95-100. doi:10.1016/S0378-8741(00)00270-1
[9] M. Delcourt, W. J. Peumans, M. C. Wanger and P. Truffa-Bachi, “V Beta Specific Deletion of Mature Thymocytes Induced by the Plant Superantigen Urtica dioica agglutinin,” Cellular Immunology, Vol. 168, No. 2, 1996, pp. 158-164. doi:10.1006/cimm.1996.0062
[10] P. Musette, A. Galelli and H. Harbre, “Urtica dioica Agglutinin, a V Beta 8.3-Specific Superantigen, Prevents the Develop Mentor the Systemic Lupus Erythematosus like Pathology of MRL Ipr/Ipr Mice,” European Journal of Immunology, Vol. 26, No. 8, 1996, pp. 1707-1711. doi:10.1002/eji.1830260807
[11] A. A. Basaran, I. Ceritoglu, U. Undeger and N. Basaran, “Immunomodulatory Activities of Some Turkish Medicinal Plants,” Phytotherapy Research, Vol. 11, No. 8, 1997, pp. 609-611. doi:10.1002/(SICI)1099-1573(199712)11:8<609::AID-PTR165>3.0.CO;2-0
[12] P. Rovira, M. Buckle, J. P. Abastado, W. J. Peumans and P. Truffa-Bachi, “Major Histocompatibility Class I Molecules Present Urtica dioica agglutinin, a Superrantiden of Vegetalorigin to Lymphocytes,” European Journal of Immunology, Vol. 29, No. 5, 1999, pp. 1571-1580. doi:10.1002/(SICI)1521-4141(199905)29:05<1571::AID-IMMU1571>3.0.CO;2-X
[13] S. Chrubasik and E. Eisenberg, “Treatment of Rheumatic Pain with Medicine in Europe. Part 2,” Urtica dioica L. Pain Clinic, Vol. 11, No. 3, 1999, pp. 179-185.
[14] C. Randall, K. Mccthan, H. Randall and F. Dobbs, “Nettle Sting of Urtica dioica for Joint Pain: An Exploratory Study of This Complementary Therapy,” Complementary Therapies in Medicine, Vol. 7, No. 3, 1999, pp. 126-131. doi:10.1016/S0965-2299(99)80119-8
[15] E. Sezika, E. Ye?iladaa, G. Hondab, Y. Takaishic, Y. Takedad and T. Tanaka, “Traditional Medicine in Turkey X. Folk Medicine in Central Anatolia,” Journal of Ethnophar-macology, Vol. 75, No. 2-3, 2001, pp. 95-115. doi:10.1016/S0378-8741(00)00399-8
[16] E. Sezik, E. Yesilada, M. Tabata, G. Honda, Y. Takaishi, T. Fujita, T. Tanaka and Y. Takeda, “Traditional Medicine in Turkey VIII. Folk Medicine in East Anatolia,” Economic Botany, Vol. 51, No. 3, 1997, pp. 195-211. doi:10.1007/BF02862090
[17] E. Yesilada, G. Honda, E. Sezik, M. Tabata, K. Goto and Y. Lkeshiro, “Traditional Medicine in Turkey IV. Folk Medicine in Mediterranean Subdivision,” Journal of Ethnopharmacology, Vol. 39, No. 1, 1993, pp. 31-38. doi:10.1016/0378-8741(93)90048-A
[18] A. A. Basaran, I. Ceritoglu and U. Undeger, “Immuno-modulatory Activities of Some Turkish Medicinal Plants,” Phytotherapy Research, Vol. 11, No. 8, 1997, pp. 609-611. doi:10.1002/(SICI)1099-1573(199712)11:8<609::AID-PTR165>3.0.CO;2-0
[19] A. A. Lebedev, E. A. Batakov and V. A. Kurkin, “The Antioxidative Activity of a Complex Hepatoprotective Preparation,” silybokhol Rastitel’nye Resursy, Vol. 37, 2001, pp. 69-75.
[20] D. A. Johansen, “Plant Microtechnique,” 1st Edition, MC Graw Hill Book Co. Inc., New York and London, 1940, pp. 15-93.
[21] K. Peach and M. V. Tracy, “Modern Methods of Plant Analysis, Vol III and IV,” Springer Heidelberg, Berlin 1955, pp. 258-261.
[22] Anonymous, “Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare,” Controller of Publications, New Delhi, 2007, p. 191.
[23] Anonymous, “Official Methods of Analysis (AOAC), Association of Official Chemists,” 4th Edition, 4th Edition, Washington, D.C. USA, 1984, pp. 187-188.
[24] Anonymous, “Ayurvedic Pharmacopeoeia of India, Part I, Vol I. Department of Health, Ministry of Health and Family Welfare,” Government of India, New Delhi, 2004, pp. 152-165.
[25] S. A. Al-Nooman, O. Ela-Segaey and A. Ab-Allah, “Experimental Study of Antioxidant and Hepatoprotective Effects of Clove and Cardamom in Ethanol Induced Hepatotoxicity,” Tanta Medical Sciences Journal, Vol. 2, No. 1, 2007, pp. 27-36.
[26] M. Kessler, G. Ubeaud and L. Jung, “Anti- and Pro-Oxidant Activity of Rutin and Quercetin Derivatives,” Journal of Pharmacy and Pharmacology, Vol. 55, No. 1, 2003, pp. 131-142. doi:10.1211/002235702559
[27] F. Shahidi and W. P. K. J. P. D. Anasundara, “Phenolic Antioxidants,” Critical Reviews in Food Science and Nutrition, Vol. 32, No. 1, 1992, pp. 67-103. doi:10.1080/10408399209527581
[28] Koleva II, T. A. Van Beek, J. P. H. Linssen, A. de Groot and L. N. Evstatieva, “Screening of Plant Extracts for Antioxidant Activity: A Comparative Study on Three Testing Methods,” Phytochemical Analysis, Vol. 13, No. 1, 2002, pp. 8-17. doi:10.1002/pca.611

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.