Protoporphyrin IX photobleaching of subcellular distributed sites of leukemic HL60 cells based on ALA-PDT in vitro

Kangqiang Huang, Li Chen, Shunfeng Lv, Jianwen Xiong

Department of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou, China.
Laboratory of Quantum Information Technology, School of Physics and Telecommunication Engineering, South China Normal Uni- versity, Guangzhou, China.
Project Construction Center, Shantou Branch, China Mobile Group Guangdong Co., Shantou, China.

DOI: 10.4236/jbise.2012.59068                

Abstract

The leukaemia cells HL60, incubated in 10 mM/ml ALA (5-aminolevulinic) for 4 hours, were carried an experimental research with fluorescent probes in photodynamic therapy (PDT) based on ALA by using PDT reaction room (The average fluence rate of the 412 nm source was 5 mW/cm²). Cells viability were determined using a Cell Counting Kit-8 (CCK-8) assay, and PpIX Photobleaching of subcellular distributed sites of HL60 cells in vitro were investigated by fluorescence spectra acquired during treatment. The results showed that the fluorescence intensity of mitochondria, lysosomes, endoplasmic reticulum had decreased by 81.5%, 52.3% and 21.0%, respectively, compared with their initial values after a 45-minute light treatment. The rate of PpIX photobleaching in mitochondria was significantly higher than others. Addi-tionally, the change of the activity of HL60 cells was basically characterized by the change fluorescence intensity in mitochondria, which suggest that mitochondria is one of main therapeutic targets of photodynamic therapy.

Keywords

5-Aminolaevulinic Acid (ALA); Photodynamic Therapy (PDT); PpIX (Protoporphyrin IX); Photobleaching; Subcellular

Conflicts of Interest

The authors declare no conflicts of interest.

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