Lab-Scale Performance Evaluation of Vertical Flow Reed Beds for the Treatment of Chlorobenzene Contaminated Groundwater


Chlorinated Benzenes (CBs) that were released into the environment contaminate groundwater at many existing and former industrial sites. A research program was initiated to investigate the ecoremediation of CBs contaminated groundwater using subsurface flow constructed wetlands. Four lab-scale experiments were performed to evaluate re- moval efficiency with different operation conditions. The first experiment was achieved with two different solid-state materials: a peat and a lava stone (pozzolana). In order to stimulate biological activity, organic matter coming from an aged Vertical Flow Constructed Wetlands (VFCW) was added to the media. Mass balance was determined to assess the fate of these pollutants in this system. The biofiltres of the second experiment were constructed with the same materials but bioaugmentation was realized by adding organic matter of VFCW or by bacteria inoculums. Peat and pozzolana biofiltres planted with Phragmites australis constituted the third experiment to evaluate the effect of plants. Bioaugmen- tation was constituted by the addition of OM coming from aged VFCW. Compost mixed with pozzolana was the solid-state material of the fourth experiment. Columns were made of two stages. The first stage was unplanted and the second stage was planted with Phragmites. Peat has been replaced by compost, a renewable material. Lab-scale biofil- tres remove CBs with an efficiency of 70% - 99%, depending on studied media and conditions. Greater efficiency was observed with bioaugmented media. Volatilization was very low (<0.2%) and the detection of chlorides in water indi- cated the occurrence of biodegradation. The experiments have shown that organic solid-state materials (compost or peat) are useful for groundwater remediation, with higher treatment efficiency than pozzolana material. Bioaugmentation increased biological activity. Clogging of biofiltres have been observed and can be reduced by the presence of plants or by a resting period of 14 - 21 days (requiring alternative feedings on several filters).

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G. Merlin and N. Cottin, "Lab-Scale Performance Evaluation of Vertical Flow Reed Beds for the Treatment of Chlorobenzene Contaminated Groundwater," Journal of Environmental Protection, Vol. 3 No. 8A, 2012, pp. 847-855. doi: 10.4236/jep.2012.328099.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] A. A. Meharg, J. Wright and D. Osborn, “Chlorobenzenes in Rivers Draining Industrial Catchments,” Science of The Total Environment, Vol. 251-252, 2000, pp. 243-253. doi:10.1016/S0048-9697(00)00387-9
[2] V. Elango, J. Cashwell and M. Bellotti, “Bioremediation of Hexachlorocyclohexane Isomers, Chlorinated Benzenes, and Chlorinated Ethenes in Soil and Fractured Dolomite,” Bioremediation Journal, Vol. 14, No. 1, 2010, pp. 10-27. doi:10.1080/10889860903463695
[3] Y. Song, F. Wang and Y. Bian, “Chlorobenzenes and Organochlorinated Pesticides in Vegetable Soils from an Industrial Site, China,” Journal of Environmental Sciences-China, Vol. 24, No. 3, 2012, pp. 362-368. doi:10.1016/S1001-0742(11)60720-1
[4] T. G. Bulc and A. S. Slak, “Ecoremediations—A New Concept in Multifunctional Ecosystem Technologies for Environmental Protection,” Desalination, Vol. 246, No. 1-3, 2009, pp. 2-10. doi:10.1016/j.desal.2008.03.039
[5] E. S. Gilbert and D. E. Crowley, “Plants Compounds That Induce Polychlorinated Biphenyl Biodegradation by Arthrobacter sp. Strain B1B,” Applied Environmental Microbiology, Vol. 63, 1997, pp. 1933-1938.
[6] M. Braeckevelt, G. Mirschel, A. Wiessner, M. Rueckert, N. Reiche and C. Vogt, “Treatment of Chlorobenzene-Contaminated Groundwater in a Pilot-Scale Constructed Wetland,” Ecological Engineering, Vol. 33, No. 1, 2008, pp. 45-53. doi:10.1016/j.ecoleng.2008.02.002
[7] M. Braeckevelt, M. Reiche, N, Trapp, S. Wiessner, A. Paschke, H. Kuschka and M. Kaestner, “Chlorobenzene Removal Efficiencies and Removal Processes in a Pilot-Scale Constructed Wetland Treating Contaminated Groundwater,” Ecological Engineering, Vol. 37, No. 6, 2011, pp. 903-913. doi:10.1016/j.ecoleng.2011.01.014
[8] G. Imfeld, M. Braeckevelt, P. Kuschk and H. H. Richnow, “Monitoring and Assessing Processes of Organic Chemicals Removal in Constructed Wetlands,” Chemosphere, Vol. 74, No. 3, 2009, pp. 349-362. doi:10.1016/j.chemosphere.2008.09.062
[9] X. Tang, P. E. Eke, M. Scholz and S. Huang, “Processes Impacting of Benzene Removal in Vertical-Flow Constructed Wetlands,” Bioresource Technology, Vol. 100, No. 1, 2009, pp. 227-234. doi:10.1016/j.biortech.2008.05.038
[10] T. F. Guerin, “Ex-Situ Bioremediation of Chlorobenzenes in Soil,” Journal of Hazardous Materials, Vol. 154, 2008, pp. 9-20. doi:10.1016/j.jhazmat.2007.09.094
[11] P. Molle, A. Lienard, C. Boutin, G. Merlin and A. Ywema, “How to Treat Raw Sewage with Constructed Wetlands: An Overview of the French Systems,” Water Science and Technology, Vol. 9, 2005, pp. 11-21.
[12] J. A. Field and R. Sierra-Alvarez, “Microbial Degradation of Chlorinated Benzenes,” Biodegradation, Vol. 19, No. 4, 2008, pp. 463-480. doi:10.1007/s10532-007-9155-1
[13] N. Cottin and G. Merlin, “Fate of Chlorinated Benzenes in Laboratory Peat and Pozzolana Filters,” Water, Air & Soil Pollution, Vol. 213, No. 1-4, 2010, pp. 425-435. doi:10.1007/s11270-010-0396-y
[14] C. Rimington, “The Carbohydrate Complex of the Serumproteins. II: Improved Method for Isolation and Redetermination of Structure. Isolation of Glucosamino Dimannose from Protein of Oxblood,” Biochemical Journal, Vol. 25, 1931, pp. 1062-1071.
[15] M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, “Colorimetric Method for Determination of Sugars and Related Substances,” Analytical Chemistry, Vol. 28, 1956, pp. 350-356.
[16] T. W. Fraser and A. Gilmour, “Scanning Electron Micro- scopy Preparation Methods: Their Influence on the Mor- phology and Fibril Formation in Pseudomonas fragi.,” Journal of Applied Bacteriology, Vol. 60, 1986, pp. 527- 533. doi:10.1111/j.1365-2672.1986.tb01092.x
[17] N. Reiche, W. Lorenz and H. Borsdorf, “Development and Application of Dynamicair Chambers for Measure- ment of Volatilization Fluxes of Benzene and MTBE from Constructed Wetlands Planted with Commonreed,” Chemosphere, Vol. 79, No. 2, 2010, pp. 162-168. doi:10.1016/j.chemosphere.2010.01.017
[18] S. Vainberg, C. Condee and S. Robert, “Large-Scale Production of Bacterial Consortia for Remediation of Chlorinated Solvent-Contaminated Groundwater,” Jour- nal of Industrial Microbiology & Biotechnology, Vol. 36, No. 9, 2009, pp. 1189-1197. doi:10.1007/s10295-009-0600-5
[19] B. Vu, M. Chen and R. J. Crawford, “Bacterial Extracellu- lar Polysaccharides Involved in Biofilm Formation,” Molecules, Vol. 14, No. 7, 2009, pp. 2535-2554. doi:10.3390/molecules14072535
[20] T. Narancic, L. Djokic, S. T. Kenny, K. E. O’Connor, V. J. Radulovic, J. Nikodinovic-Runi and B. Vasiljevic, “Metabolicversatility of Gram-Positive Microbialisolates from Contaminated River Sediments,” Journal of Hazar- dous Materials, Vol. 215-216, 2012, pp. 243-251. doi:10.1016/j.jhazmat.2012.02.059

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