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Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

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DOI: 10.4236/jsemat.2012.223032    4,485 Downloads   6,946 Views   Citations

ABSTRACT

We demonstrated the insitu observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Yanagiya and N. Goto, "Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal," Journal of Surface Engineered Materials and Advanced Technology, Vol. 2 No. 3A, 2012, pp. 210-214. doi: 10.4236/jsemat.2012.223032.

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