Crithidia deanei infection in normal and dexamethasone–immunosuppressed Balb/c mice

Dilvani Oliveira Santos, Saulo C. Bourguignon, Helena Carla Castro, Alice Miranda, Rodrigo Tonioni Vieira, Suzana Corte-Real, Otílio Machado Pereira Bastos

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DOI: 10.4236/health.2010.26087                

Abstract

Monoxenous trypanossomatids protozoa are not believed to cause in vivo infection in verte- brate hosts throughout their life cycle. However, there are reports mentioning some cases of HIV- positive patients who have presented opportunistic infections caused by these protozoa. Recently, we have demonstrated the in vitro infection of mouse dermal fibroblasts by these protozoa. The aim of the present work is to investigate the possibility of Crithidia deanei, a endosymbiont-bearing monoxenous trypanossomatid, infect BALB/c mice under or not Dexamethasone treatment. To attend it, distinct gro- ups of adult BALB/c mice were immunosuppressed with 50 mg/kg of Dexamethasone. This immunosuppressor was administered 24 hours before infection and daily, for 15 days after C. deanei inoculation. Control groups: C. deanei–inoculated animals but non-immuno- suppressed and non-inoculated animals but immunosuppressed were also used. Light Microscopy analysis revealed an infection process characterized by the presence of the trypanossomatid inside dermal cells in the groups studied. The experimental inoculation resulted in a non-lethal infection characterized by the presence of the trypanossomatid inside dermal cells in the normal BALB/c mice, but notably, in the C. deanei–inoculated immunosuppressed group. These preliminary results lead to the following conclusions: 1) C. deanei is able to infect normal BALB/c mice; 2) the immunosupressed mice seemed to be more susceptible to the C. deanei infection compared to the control group. Besides C. deanei in dexamethasone-immunosuppressed mice provides a useful model for studies of monoxenous trypanosomatids ‘in vivo’ infection, resembling that one presumably occurring in imunodeficient individuals with AIDS.

Keywords

Monoxenous Trypanossomatid;‘In Vivo’ Infection; Immunosuppression

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Vickermank, K. (1994) The evolutionary expansion of the trypanossomatid flagellates. International Journal for Parasitology, 24(8), 1317-1331.
[2]	Wallace, F. G. (1966) The trypanosomatid parasites of insects and arachnids. Experimental Parasitology, 18(1), 124-193.
[3]	Pacheco, R.S., Marzochi, M. and Pires, M. (1998) Parasite genotypically related to a monoxenous trypnosoamtid of dog’s flea causing opportunistic infection in HIV- positive patient. Memórias do Instituto Oswaldo Cruz, 93 (4), 531-537.
[4]	Boisseau–Garsaud, A.M., Cales-Quist, D. and Desbois, N. (2000) A new case of cutaneous infection by a presumed monoxenous trypanosomatid in the island of Martinique (French WEst Indies). Transactions of the Royal Society of Tropical Medicine and Hygiene, 94(1), 51-52.
[5]	Santos, D.O., Bourguignon, S.C. and Castro, H. C. (2004) Infection of mouse dermal fibroblasts by the monoxenous trypanosomatid Protozoa Crithidia deanei and Her- petomonas roitmani. Journal of Eukaryotic Microbiology, 51, 570-574.
[6]	Noyes, P., Pratlong, F. and Chance, M. (2002) A previously unclassified trypnosomatid responsible for human cutaneous lesions in Martinique (French West Indies) is the most divergent member of the genus Leishmania ss. Parasitology, 124(Pt 1), 17-24.
[7]	Warren, L.G. (1960) Metabolism of schizotrypanumcruzi Chagas. 1. Effect of culture age and substrate concentration on respiratory rate. Journal of Parasitology, 46, 529- 539.
[8]	Lallo, M.A., Santos, M.J. and Bondam, E.F. (2002) Experimenatal encephalitozoon cuniculi infection in dexamethasone-immunosuppressed mice. Revista de Saúde Pública. 36(5), 621-626.
[9]	Santos, D.O, Castro, H.C. and Bourguignon, S.C. (2007) Expression of B7-1 costimulatory molecule in patients with multibacillary-leprosy and reactional states. Clinical and Experimental Dermatology, 32(1), 75-80.
[10]	Jiménez, M.L., lópez-Vélez, R. and Molina, R. (1996) HIV coinfection with a currently non-pathogenic flagellate. Lancet, 347(8996), 264-265.
[11]	Rozenthal, S., De Carvalho, T.U. and De Souza, W. (1987) Influence of the endosymbiont on the interaction of Chrithidia deanei with macrophages. Microscopy Electron Biology Cell, 11, 167-179.
[12]	Barreto-de-Souza, V., Xavier Medeiros, T. and Machado Motta, M.C. (2008) HIV-1 infection and HIV-1 Tat protein permit the survival and replication of a non-pathogenic trypanosomatid in macrophages through TGF beta 1 production. Microbes and Infection, 10(6), 642-649.
[13]	Podlipaev, S.A. (2001) The more insect trypanosomatids under study-the more diverse Trypanosomatidae appears. International Journal for Parasitology, 31(5-6), 648-652.
[14]	Podlipaev, S.A., Strurm, N.R. and Fiala, I. (2004) Diversity of insect trypanossomatids assessed from the splice leader RNA and 5S rRNA genes and intergenic region. Journal of Eukaryotic Microbiology, 51(3), 283- 290.
[15]	Diasio, R.B. and LoBuglio, A.F. (1996) Immunomodulators: Immunosuppressive agents and immunostimulants the pharmacological basis of therapeutics. 9th Edition, Mcgraw Hill, London.
[16]	Ghalib Ghalib, H.W., Piuvezam, M.R. and Skeiky, Y.A.W. (1993) Interleukin 10 production correlates with pathology in human Leishmania donovani infections. Journal of Clinical Investigation, 92(1), 324-329.
[17]	Carvalho, E.M., Bacellar, O. and Brownell, C. (1994) Restoration of the IFN-g production and lymphocyte proliferation in visceral leishmaniasis. Journal of Immunology, 152(12), 5949-5956.
[18]	Mary, C., Lamouroux, D. and Dunan, S. (1992) Western blot analysis of antibodies to Leishmania infantum antigens: potencial of the 14-KD and 16–KD antigens for diagnosis and epidemiologic purposes. American Journal of Tropical Medicine and Hygiene, 47(6), 764-771.
[19]	Matteoli, F.P., D’Avila-Levy, C.M. and Santos, L.O. (2009) Roles of the endosymbiont and leishmanolysin-like molecules expressed by Crithidia deanei in the interaction with mammalian fibroblasts. Experimental Parasitology, 121(3), 246-253.
[20]	Corrêa-da Silva, M., Fampa, P. and Lessa, L.P. (2006) Colonizatin of Aedes aegypti midgut by the endosymbiont-bearing trypanosomatid Blastocrithidia culicis. Parasitology Research, 99(4), 384-391.
[21]	D’Avila-Levy, C.M., Santos, L.O., Marinho, F.A. and Matteoli, F.P. (2008) Crithidia deanei: Influence of parasite gp63 homologue on the interaction of endosymbi- ont–harbouring and aposymbiotic strains with Aedes aegypty midgut. Experimental Parasitology, 118(3), 345- 353.