Share This Article:

A study of aqueous humour proteins in patients of primary open angle glaucoma

Abstract Full-Text HTML XML Download Download as PDF (Size:291KB) PP. 110-114
DOI: 10.4236/abb.2010.12015    4,860 Downloads   9,721 Views   Citations

ABSTRACT

Aims: To investigate the changes in the protein composition of aqueous humour in primary open angle glaucoma patients and non-glaucomatous subjects. Patients and Methods: Case control study was conducted at a university hospital to compare the protein profile of aqueous humour obtained from patients of primary open angle glaucoma with those of non glaucomatous subjects. Protein concentration of the aqueous humour samples collected from both glaucomatous and non-glaucomatous patients at the time of surgery was estimated by the Bradford protein assay method. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the samples was also performed. Results: Geometric mean of protein concentration of the samples was 55.73 mg/dl (range: 31-72) in the test group and 46.46 mg/dl (range: 27-65) in the control group and a statistically significant (p ≤= 0.001) difference in protein concentration of aqueous humour between the two groups was observed. Electrophoretic study revealed differences in the aqueous protein composition of the two groups. The glaucomatous group exhibited protein bands of 10 kDa, 20 kDa, 30 kDa, 50 kDa, 60 kDa, 70 kDa, and 90 kDa while the control subjects demonstrated bands of 6 kDa, 10 kDa, 30 kDa, 70 kDa, 90 kDa. Interestingly, almost all the proteins detected in this study corresponded to the molecular weights of heat shock proteins. Conclusions: Primary open angle glaucoma patients differed in protein compositions and had higher concentration of aqueous humour proteins than non glaucomatous patients.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Zaidi, M. , Jilani, A. , Bhattacharya, P. , Islam, N. and Alam, S. (2010) A study of aqueous humour proteins in patients of primary open angle glaucoma. Advances in Bioscience and Biotechnology, 1, 110-114. doi: 10.4236/abb.2010.12015.

References

[1] Sommer, A., Tielsch, J.M., Katz, J., et al. (1991) Relationship between intraocular pressure and primary open angle glaucoma among white and black patients: the Baltimore Eye Survey. Archives of Ophthalmology, 109(8), 1090-1095.
[2] Quigley, H.A. and Broman, A.T. (2006) The number of people with glaucoma worldwide in 2010 and 2020. British Journal of Ophthalmology, 90(3), 262-267.
[3] Quigley, H.A. (1996) Number of people with glaucoma worldwide. British Journal of Ophthalmology, 80(5), 389393.
[4] Prata, T.S., Navajos, E.V., Melo, L.A., Jr., et al. (2007) Aqueous humor protein concentration in patients with primary open angle glaucoma under clinical treatment. Arquivos Brasileiros de Oftalmologia, 70(2), 217.
[5] Saari, K.M., Aine, E. and Parvianen, M. (1983) Determination of protein content in aqueous humour by highperformance gel filtration chromatography. Acta Ophthalmologia (Copenh), 61(4), 611-617.
[6] Inada, K., Muarata, T., Baba, H., et al. (1988) Increase of aqueous humor proteins with aging. Japanese Journal of Ophthalmology, 32(2), 126-131.
[7] Tripathi, R.C., Millard, C.B. and Tripathi, B.J. (1989) Protein composition of human aqueous humor: SDS- PAGE analysis of surgical and post-mortem samples. Experimental Eye Research, 48(1), 117-130.
[8] Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254.
[9] Ladas, J.G., Fei, Y., Loo, R., et al. (2001) Relationship between aqueous humour protein level and outflow facility in patients with uveitis. Investigative Ophthalmology and Visual Science, 42(11), 2584-2588.
[10] Stur, M., Grabner, G., Huber-Spitzy, V., Schreiner, J. and Haddad, R. (1986) Effect of timolol on aqueous humor protein concentration in the human eye. Archives of Ophthalmology, 104(6), 899-900.
[11] Beardsley, T.L. and Shields, M.B. (1983) Effect of timolol on aqueous humor protein concentration in humans. American Journal of Ophthalmology, 95(4), 448450.
[12] Oshika, T. and Araie, M. (1990) Time course of changes in aqueous protein concentration and flow rate after oral acetazolamide. Investigative Ophthalmology and Visual Science, 31(3), 527-534.
[13] Shahid, H., Farahdiba, J. and Crosson, C.E. (2005). Acute effects of PGF2α on MMP-2 secretion from human ciliary muscle cells: A PKCand ERK-dependent process. Investigative Ophthalmology and Visual Science, 46(5), 17061713.
[14] Herschler, J. and Litin, B.S. (1987) Biochemical abnormalities in the aqueous in chronic open angle glaucoma. Ophthalmic Surgery, 18(11), 792-795.
[15] Lee, I.S., Yu, Y.S., Kim, D.M., et al. (1990) Detection of specific proteins in the aqueous humor in primary open angle glaucoma. Korean Journal of Ophthalmology, 4(1), 1-4.
[16] Bagchi, M., Katar, M. and Maisel, H. (2002) Effect of exogenous stress on the tissue-cultured mouse lens epithelial cells. Journal of Cell Biochemistry, 86(2), 302-306.
[17] Banh, A., Vijayan, M.M. and Sivak, J.G. (2003) Hsp70 in bovine lenses during temperature stress. Molecular Vision, 9, 323-328.
[18] Barbe, M.F., Tytell, M., Gower, D.J. and Welch, W.J. (1988) Hyperthermia protects against light damage in the rat retina. Science, 241(4874), 1817-1820.
[19] Tanaka, Y., Kobayashi, K., Kita, M., et al. (1996) Expression of 47 kDa heat shock protein (Hsp47) during development of mouse cornea. Experimental Eye Research, 63(4), 383-393.
[20] Tamm, E.R., Russell, P., Johnson, D.H. and Piatigorsky, J. (1996) Human and monkey trabecular meshwork accumulate alpha B-crystallin in response to heat shock and oxidative stress. Investigative Ophthalmology and Visual Science, 37(12), 2402-2413.
[21] Yao, K., Rao, H., Wu, R., et al. (2006) Expression of Hsp70 and Hsp27 in lens epithelial cells in contused eye of rat modulated by thermotolerance or quercetin. Molecular Vision, 12, 445-450.
[22] Tripathi, R.C., Borisuth, N.S., Tripathi, B.J., et al. (1992) Quantitative and qualitative analysis of transferrin in aqueous humor from patients with primary and secondary glaucoma. Investigative Ophthalmology and Visual Science, 33(10), 2866-2873.
[23] Wax, M. (2001) Serum autoantibodies to heat shock proteins in glaucoma patients from Japan and the United States. Ophthalmology, 108(2), 296-302.
[24] Tezel, G., Hernandez, R. and Wax, M.B. (2000) Immunostaining of heat shock proteins in the retina and optic nerve head of normal and glaucomatous eyes. Archives of Ophthalmology, 118(4), 511-518.
[25] Dan, J., Belyea, D., Gertner, G., et al. (2005) Plasminogen activator inhibitor-1 in the aqueous humor of patients with and without glaucoma. Archives of Ophthalmology, 123(2), 220-224.
[26] Wang, Y., Taylor, D.M., Smalley, D.M., Cone, R.E. and O'Rourke, J. (1994) Increased basal levels of free plasminogen activator found in human aqueous humor. Investigative Ophthalmology and Visual Science, 35(9), 35613566.
[27] Bernatchez, S.F., Tabatabay, C. and Belin, D. (1992) Urokinase-type plasminogen activator in human aqueous humor. Investigative Ophthalmology and Visual Science, 33(9), 2687-2692.
[28] Lee, S., Carson, K., Rice-Ficht, A. and Good, T. (2005) Hsp20, a novel α-crystallin, prevents A β fibril formation and toxicity. Protein Science, 14(3), 593-601.
[29] Doss, E.W., Ward, K.A. and Koretz, J.F. (1998) Investigation of the “fines” hypothesis of primary open angle glaucoma: The possible role of alpha-crystallin. Ophthalmic Research, 30(3), 142-156.
[30] Ethier, C.R., Kamm, R.D., Johnson, M., et al. (1989) Further studies on the flow of aqueous humor through microporous filters. Investigative Ophthalmology and Visual Science, 30(4), 739-746.
[31] Pavao, A.F., Lee, D.A., Ethier, C.R., et al. (1989) Twodimensional gel electrophoresis of calf aqueous humor, serum, and filter-bound proteins. Investigative Ophthalmology and Visual Science, 30(4), 731-738.

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.