Introgression the Salinity Tolerance QTLs Saltol into AS996, the Elite Rice Variety of Vietnam

Abstract

This study focus on developing new salinity tolerance and high yielding rice lines, using markers assisted backrossing (MABC). Total of 500 SSR markers on 12 rice chromosomes were screened for parental polymorphic markers. Of which, 52 primers in the Saltol region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol region of the breeding populations. For each backcross generation of ASS996/FL478, approx. 500 plants were screened with 63 polymorphic markers distributed on 12 chromosomes. The two BC1F1 plants P284 and P307 which had the highest recipient alleles up to 89.06% and 86.36%, were chosen for the next backcrossing. Three BC2F1 plants with the recipient alleles up to 94.03% and 93.18% were used to develop BC3F1 generation. The best BC3F1 plant was P284-112-209 with all the recipient alleles and Saltol region. The four plants P307-305-21, P284-112-195, P284-112-198, P284-112-213 were the second ranking with only one loci heterozygous (applied 63 markers covered on 12 chromosomes). These five plants were chosen as the breeding lines for result of Saltol-AS996 introgression. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new salinity tolerant variety ASS996-Saltol to cope with climate change.

Share and Cite:

L. Huyen, L. Cuc, A. Ismail and L. Ham, "Introgression the Salinity Tolerance QTLs Saltol into AS996, the Elite Rice Variety of Vietnam," American Journal of Plant Sciences, Vol. 3 No. 7, 2012, pp. 981-987. doi: 10.4236/ajps.2012.37116.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Thomson MJ, Ismail AM, McCouch SR, Mackill MJ. Marker assisted breeding. In: Pareek A, Sopory SK, Bohnert HJ, Govindjee, editors. Abiotic stress adaptation in plants: physiological, molecular and genomic foundation. New York: Springer; (2010). p. 451–69.
[2] Septiningsih EM, Pamplona AM, Sanchez DL, Neeraja CN, Vergara GV, Heuer S, Ismail AM, Mackill DJ (2009) Development of submergence tolerant rice cultivars: the Sub1 locus and beyond. Ann. Bot. 103:151–160.
[3] Singh RK, Redo?a ED, Refuerzo L. Varietal improvement for abiotic stress tolerance in crop plants: special reference to salinity in rice. In: Pareek A, Sopory SK, Bohnert HJ, Govindjee, editors. Abiotic stress adaptation in plants: physiological, molecular and genomic foundation. New York: Springer; (2010). p. 387–415.
[4] Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the 21st century. Phil Trans Royal Soc. B. Rev. 363:557–572.
[5] Kim S-H, Bhat PR, Cui X, Walia H, Xu J, Wanamaker S, et al. Detection and validation of single feature polymorphisms using RNA expression data from a rice genome array. BMC Plant Biol. (2009);9:65.
[6] Van Berloo R (2008) GGT 2.0: versatile software for visualization and analysis of genetic data. J Hered 99:232–236.
[7] Yoshida S, Forno DA, Cock JK, Gomez KA. Laboratory manual for physiological studies of rice. Manila: International Rice Research Institute; (1976). p. 38.
[8] IRRI. Standard evaluation system for rice. 4th ed. Manila: International Rice Research Institute; (1996). p. 52.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.