Cytotoxicity of Pristine C₆₀ Fullerene on Baby Hamster Kidney Cells in Solution

Shufang Liu, Haijie Liu, Zhijuan Yin, Kai Guo, Xibao Gao

School of Public Health, Shandong University, Jinan, 250012, P. R. China.

DOI: 10.4236/jbnb.2012.33037                

Abstract

Research on biological effects of C₆₀ and its derivatives is one of the hotspots in the area of biological effects of nanomaterials. Compared with surface-modified C₆₀ derivatives, reports on the biological effects of unmodified pristine C₆₀ are relatively less. This work aimed to investigate the interaction between baby hamster kidney cells and pristine C₆₀ in solution. The C₆₀ suspension was prepared using solvent exchange method and characterized by UV spectrophotometry, electronic transmission microscopy and dynamic light scattering techniques. The baby hamster kidney cells were incubated with different concentrations of C₆₀ suspensions, and light microscopy, cell counting kit 8 assay, and acridine orange staining were used to observe the cell growth and morphology. The results showed that C₆₀ could ihibit the cell growth and induce cell apoptosis with a dose-effect relationship. C₆₀ might enter cells and the possible way it enters cells were also proposed.

Keywords

C₆₀; Cell; Cytotoxicity; Fullerene; Toxicity;

Conflicts of Interest

The authors declare no conflicts of interest.

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