Sustainable Discoloration of Textile Chromo-Baths by Spent Mushroom Substrate from the Industrial Cultivation of Pleurotus ostreatus

DOI: 10.4236/jep.2010.12011   PDF   HTML     6,135 Downloads   11,129 Views   Citations


Synthetic dyes are recalcitrant to degradation and toxic to different organisms. Physical-chemical treatments of textile wastewaters are not sustainable in terms of costs. Biological treatments can be more convenient and the lig-nin-degrading extracellular enzymatic battery of basidiomycetes are capable to discolor synthetic dyes. Many basidi-omycetes are edible mushrooms whose industrial production generates significant amount of spent mushroom substrate (SMS) with residual high levels of lignin-degrading extracellular enzymatic activities. We have demon-strated that the low cost organic substrate, the SMS deriving from the cultivation of the basidiomycetes Pleurotus ostreatus, is able to discolor anthraquinonic, diazo and monoazo-dyes when incubated in dying chromo-reactive and chromo-acid baths containing surfactants and anti-foams, where the concentrations of the different dyes are exceeding the one recovered in the corresponding wastewaters. Laccase was the lignin-degrading extracellular enzyme involved in the discolouring process. The exploitation of the low cost SMS in the treatment of textile wastewaters is proposed. Accordingly, a toxicological assessment, based on a cyto-toxicity test on a human amnion epithelial cell line (WISH) and the estimation of the germination index (GI%) of Lactuca sativa, Cucumis sativus and Sorghum bicolor, has been performed, showing the loss of toxicity of the chromo-baths after being discoloured by the SMS.

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S. Gregorio, F. Balestri, M. Basile, V. Matteini, F. Gini, S. Giansanti, M. Tozzi, R. Basosi and R. Lorenzi, "Sustainable Discoloration of Textile Chromo-Baths by Spent Mushroom Substrate from the Industrial Cultivation of Pleurotus ostreatus," Journal of Environmental Protection, Vol. 1 No. 2, 2010, pp. 85-94. doi: 10.4236/jep.2010.12011.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] T. Deveci, A. Unyayar and M. A. Mazmanci, “Production of Remazol Brilliant Blue R Decolourising Oxygenase from the Culture Filtrate of Funalia Trogii ATCC 200800,” Journal of Molecular Catalysis B: Enzymatic, Vol. 30, No. 1, 2004, pp. 25-32.
[2] K. P. Sharma, S. Sharma, S. Sharma, P. K. Singh, S. Ku-mar, R. Grover and P. K. Sharma, “A Comparative Study on Characterization of Textile Wastewaters (Untreated and Treated) Toxicity by Chemical and Biological Tests,” Chemosphere, Vol. 69, No. 1, 2007, pp. 48-54.
[3] I. M. Banat, P. Nigam, D. Singh and R. Marchant, “Mi-crobial Decolourization of Textile Dyes Containing Ef-fluents: A Review,” Bioresource Technology, Vol. 58, No. 3, 1996, pp. 217-227.
[4] A. Stolz, “Basic and Applied Aspects in the Microbial Degradation of Azo Dyes,” Applied Microbiology and Biotechnology, Vol. 56, No. 1-2, 2001, pp. 69-80.
[5] D. Brown and B. Hamburger, “The Degradation of Dye-stuffs: Part III—Investigation of their Ultimate Degrad-ability,” Chemosphere, Vol. 16, No. 7, 1987, pp. 1539- 1553.
[6] A. Gottlieb, C. Shaw, A. Smith, A. Wheatley and S. Forsythe, “The Toxicity of Textile Reactive Azo Dyes after Hydrolysis and Decolourisation,” Journal of Bio-technology, Vol. 101, No. 1, 2003, pp. 49-56.
[7] J. A. Libra, M. Borchert, L. Vigelahn and T. Storm, “Two Stage Biological Treatment of a Diazo Reactive Textile Dye and the Fate of the Dye Metabolites,” Chemosphere, Vol. 56, No. 2, 2004, pp. 167-180.
[8] J. S. Knapp, P. S. Newby and L. P. Reece, “Decolorization of Dyes by Wood-Rotting Basidiomycete Fungi,” Enzyme and Microbial Technology, Vol. 17, No. 7, 1995, pp. 664-668.
[9] D. Wesenberg, I. Kyriakides and S. N. Agathos, “White- Rot Fungi and their Enzymes for the Treatment of Indus-trial Dye Effluents,” Biotechnology Advances, Vol. 22, No. 1-2, 2003, pp. 161-87.
[10] E. P. Chagas and L. R. Durrant, “Decolorization of Azo Dyes by Phanerochaete Chrysosporium and Pleurotus Sajorcaju,” Enzyme and Microbial Technology, Vol. 29, No. 8, 2001, pp. 473-477.
[11] M. A. Martins, N. Lima, A. J. Silvestre and M. J. Queiroz, “Comparative Studies of Fungal Degradation of Single or Mixed Bioaccessible Reactive Azo Dyes,” Chemosphere, Vol. 52, No. 6, 2003, pp. 967-973.
[12] J. Swamy and J. A. Ramsay, “The Evaluation of White Rot Fungi in the Decoloration of Textile Dyes,” Enzyme and Microbial Technology, Vol. 24, No. 3-4, 1999, pp. 130-137.
[13] A. Heinfling, M. Bergbauer and U. Szewzyk, “Biodegra-dation of Azo and Phthalocyanine Dyes by Trametes Ver-sicolor and Bjerkandera adusta,” Applied Microbiology and Biotechnology, Vol. 48, No. 2, 1997, pp. 261-266.
[14] G. Aggelis, D. Iconomou, M. Christou, D. Bokas, S. Kotzailias, G. Christou, V. Tsagou and S. Papanikolaou, “Phenolic Removal in a Model Olive Oil Mill Wastewater Using Pleurotus Ostreatus in Bioreactor Cultures and Biological Evaluation of the Process,” Water Research, Vol. 37, No. 16, 2003, pp. 3897-3904.
[15] S. Rodríguez-Pérez, N. G. Oduardo, R. C. B. Savón, M. F. Boizán and C. Augur, “Decolourisation of Mushroom Farm Wastewater by Pleurotus Ostreatus,” Biodegradation, Vol. 19, No. 4, 2008, pp. 519-526.
[16] L. H. Zhao, J. T. Zhou, H. Lu, C. L. Zheng, Y. S. Yang, H. J. Sun and X. H. Zhang, “Decolorization of Cotton Pulp Black Liquor by Pleurotus Ostreatus in a Bubble- Column Reactor,” Bulletin of Environmental Contamination and Toxicology, Vol. 80, No. 1, 2008, pp. 44-48.
[17] V. Faraco, C. Pezzella, A. Miele, P. Giardina and G. Sannia, “Bio-Remediation of Colored Industrial Waste-waters by the White-Rot Fungi Phanerochaete Chrysos-porium and Pleurotus Ostreatus and their Enzymes,” Biodegradation, Vol. 20, No. 2, 2009, pp. 209-220.
[18] A. S. Ball and A. M. Jackson, “The Recovery of Ligno-cellulose Degrading Enzymes from Spent Mushroom Compost,” Bioresource Technology, Vol. 54, No. 3, 1995, pp. 311-314.
[19] S. W. Chiu, S. C. Law, M. L. Ching, K. W. Cheung and M. J. Chen, “Themes for Mushroom Exploitation in the 21st Century: Sustainability, Waste Management, and Conservation,” Journal of General and Applied Micro- biology, Vol. 46, No. 6, 2000, pp. 269-282.
[20] M. R. Trejo Hernandez, A. L. Munguia and R. Q. Ramirez, “Residual Compost of Agaricus Bisporus as a Source of Crude Laccase for Enzymic Oxidation of Phenolic Compounds,” Process Biochemistry, Vol. 36, No. 7, 2001, pp. 635-639.
[21] M. D. Karla, T. H. Maria del Refugio and R. P. Marco, “Recovery of Laccase from the Residual Compost of Agaricus Bisporus in Aqueous Two-Phase Systems,” Process Biochemistry, Vol. 44, No. 4, 2009, pp. 435-439.
[22] H. G. Ko, S. H. Park, S. H. Kim, H. G. Park and W. M. Park, “Detection and Recovery of Hydrolytic Enzymes from Spent Compost of Four Mushroom Species,” Folia Microbiologica, Vol. 50, No. 2, 2005, pp. 103-106.
[23] W. M. Law, W. N. Lau, K. L. Lo, L. M. Wai and S. W. Chiu, “Removal of Biocide Pentachlorophenol in Water System by The Spent Mushroom Compost of Pleurotus Puulmonarius,” Chemosphere, Vol. 52, No. 9, 2003, pp. 1531-1537.
[24] M. M. Bradford, “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,” Analytical Bio-chemistry, Vol. 72, 1976, pp. 248-254.
[25] A. M. Osman, K. K. Y. Wong, S. J. Hill and A. Ferny-hough, “Isolation and the Characterization of the Degra-dation Products of the Mediator ABTS-Derived Radicals Formed upon Reaction with Polyphenols,” Biochemical and Biophysical Research Communications, Vol. 340, No. 2, 2005, pp. 597-603.
[26] C. G. Boer, L. Obici, C. Marques de Souza and R. M. Peralta, “Purification and Some Properties of Mn Peroxi-dase from Lentinula Edodes Process,” Process Biochem-istry, Vol. 41, No. 5, 2006, pp. 1203-1207.
[27] M. Tien and T. K. Kirk, “Lignin-Degrading Enzyme from Phanerochaete Chrysosporium: Purification, Characterization and Catalytic Properties of a Unique H(2)O(2)- Requiring Oxygenase,” Proceedings of the National Academy of Sciences, Vol. 81, No. 8, 1984, pp. 2280- 2284.
[28] F. Balestri, M. Giannecchini, F. Sgarrella, M. C. Carta, M. G. Tozzi and M. Camici, “Purine and Pyrimidine Nucleo-sides Preserve Human Astrocytoma Cell Adenylate Energy Charge under Ischemic Conditions,” Neurochemistry International, Vol. 50, No. 3, 2007, pp. 517-523.
[29] Y. Fu and T. Viraraghavan, “Fungal Decolorization of Dye Wastewaters: A Review,” Bioresource Technology, Vol. 79, No. 3, 2001, pp. 251-262.
[30] C. I. Pearce, J. R. Lloyd and J. T. Gurthrie, “The Removal of Colour from Textile Wastewater Using Whole Bacterial Cells: A Review,” Dyes and Pigments, Vol. 58, No. 3, 2003, pp. 179-196.
[31] D. T. D’Souza, R. Tiwari, A. K. Sah and C. Raghukumar, “Enhanced Production of Laccase by a Marine Fungus During Treatment of Colored Effluents and Synthetic Dyes,” Enzyme and Microbial Technology, Vol. 38, No. 3-4, 2006, pp. 504-511.
[32] S. Vanhulle, E. Enaud, M. Trovaslet, N. Nouaimeh, C. M. Bols, T. Keshavarz, T. Tron, G. Sannia and A. M. Cor-bisier, “Overlap of Laccases/Cellobiose Dehydrogenase Activities during the Decolourisation of Anthraquinonic Dyes with Close Chemical Structures by Pycnoporus Strains,” Enzyme and Microbial Technology, Vol. 40, No. 7, 2007, pp. 1723-1731.
[33] P. P. Champagne and J. A. Ramsay, “Contribution of Manganese Peroxidase and Laccase to Dye Decoloration by Trametes Versicolor,” Applied Microbiology and Bio-technology, Vol. 69, No. 3, 2005, pp. 276-285.
[34] H. Claus, G. Faber and F. Koneig, “Redox-Mediated Decolorization of Synthetic Dyes by Fungal Laccases,” Applied Microbiology and Biotechnology, Vol. 59, No. 6, 2002, pp. 672-678.
[35] P. Giardina, V. Farmaco, C. Pezzella, A. Piscitelli, S. Vanhulle and G. Sannia, “Laccases: A Never-Ending Story,” Cellular and Molecular Life Sciences, Vol. 67, No. 3, 2010, pp. 369-385.
[36] B. Brogioni, D. Biglino, A. Sinicropi, E. J. Reijerse, P. Giardina, G. Sannia, W. Lubitz, R. Basosi and R. Pogni, “Characterization of Radical Intermediates in Laccase- Mediator Systems. A Multifrequency EPR, ENDOR and DFT/PCM Investigation,” Physical Chemistry Chemical Physics, Vol. 10, 2008, pp. 7284-7292.
[37] E. Dubé, F. Shareck, Y. Hurtubise, M. Beauregard and C. Daneault, “Decolourization of Recalcitrant Dyes with a Laccase from Steptomyces Coelicolor under Alkaline Conditions,” Journal of Industrial Microbiology & Bio-technology, Vol. 35, No. 10, 2008, pp. 1123-1129.
[38] S. Camarero, D. Ibarra, M. J. Martinez and A. T. Martinez, “Lignin-Derived Compounds as Efficient Laccase Mediators for Decolorization of Different Types of Re-calcitrant Dyes,” Applied and Environmental Micro-biology, Vol. 71, No. 4, 2005, pp. 1775-1784.

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