Evaluation of Nitrogen and Phosphorus Wastes Produced by Nile Tilapia (Oreochromis niloticus L.) Fed Azolla-Diets in Earthen Ponds

DOI: 10.4236/jep.2012.36060   PDF   HTML     5,629 Downloads   8,232 Views   Citations


Nitrogen (N) and phosphorus (P) wastes produced by Nile tilapia Oreochromis niloticus L. fed Azolla, an aquatic atmospheric nitrogen fixing fern, was evaluated for 90 days in pond experiment. Six isonitrogenous (29.2% crude protein) and isoenergetic (16.9 Kj·g–1) diets A0, A10, A20, A30, A40 and A50, containing 0%, 10%, 20%, 30%, 40% and 50% of Azolla meal (AM) respectively, as partial fishmeal (FM) substitutes, was provided to experimental fish. The Azolla-free diet A0 served as a control. Fish specific growth rate (SGR) was higher with the control diet, the lower values being obtained in A50-fed fish (P < 0.05). Crude protein and P content in experimental fish showed similar values. Evaluation of the nutrient wasted show identical values (84.8% - 87.8% of supplied) for total P (TP); while total N (TN) discharged into ponds by fish increased significantly when AM level greater than 30% in diets (P < 0.05), amounting 63.9% - 74.2% of that supplied. From these findings, the fern Azolla could be used in diet to sustain Nile tilapia growth and as “environmentally-friendly” ingredient to limit P loss, while providing N to the field, beneficially in tropical marshland pond where this nutrient is already limiting.

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Y. Abou, A. Saidou, D. Mama, E. D. Fiogbé and J. Micha, "Evaluation of Nitrogen and Phosphorus Wastes Produced by Nile Tilapia (Oreochromis niloticus L.) Fed Azolla-Diets in Earthen Ponds," Journal of Environmental Protection, Vol. 3 No. 6, 2012, pp. 502-507. doi: 10.4236/jep.2012.36060.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] GESAMP IMO/FAO/UNESCO-IOC/WMO/WHO/IAEA/ UN/NEP (Joint Group of Experts on the Scientific Aspects of Marine Environmental Protection), “Monitoring the Ecological Effects of Coastal Aquaculture Wastes,” GESAMP, Rome, 1996.
[2] S. H. Sugiura, J. K. Babbitt, F. M. Dong and R. W. Hardy, “Utilization of Fish and Animal By-Product Meals in Low-Pollution Feeds for Rainbow Trout Onchorhnchus mykiss (Walbaum),” Aquaculture Research, Vol. 31, No. 7, 2000, pp. 585-593. doi:10.1046/j.1365-2109.2000.00476.x
[3] C. Y. Cho and D. P. Bureau, “Reduction of Waste Output from Salmonid Aquaculture Trough Feed and Feeding,” Progressive Fish-Culturist, Vol. 59, No. 2, 1997, pp. 155-160. doi:10.1577/1548-8640(1997)059<0155:ROWOFS>2.3.CO;2
[4] Y. Buyukates, S. D. Rawles and D. M. Gatlin, “III Phosphorus Fractions of Various Feedstuffs and Apparent Phosphorus Availability to Channel Catfish,” North American Journal of Aquaculture, Vol. 62, No. 3, 2000, pp. 184-188. doi:10.1577/1548-8454(2000)062<0184:PFOVFA>2.3.CO;2
[5] S. H. Sugiura and R. W. Hardy, “Environmentally Friendly Feeds,” In: R. R. Stickney, Ed., Encyclopedia of Aquaculure, Wiley-Interscience, 2000, pp. 299-310.
[6] C. Ogino, L. Takeuchi, H. Takeda and T. Watanabe, “Availability of Dietary Phosphorus in Carp and Rainbow Trout,” Nippon Suisan Gakkaishi, Vol. 45, 1979, pp. 1527-1532. doi:10.2331/suisan.45.1527
[7] B. S. Nakashima and W. C. Leggett, “Natural Sources and Requirements of Phosphorus for Fishes,” Canadian Journal of Fisheries and Aquatic Sciences, Vol. 37, No. 4, 1980, pp. 679-686. doi:10.1139/f80-085
[8] S. Zhang, S. Xie, X. Zhu, W. Lei, Y. Yang and M. Zhao, “Meat and Bone Meal Replacement in Diets for Juvenile Gibel Carp (Carasius auratus gibelio): Effects on Growth Performance, Phosphorus and Nitrogen Loading,” Aquaculture Nutrition, Vol. 12, No. 5, 2006, pp. 353-362. doi:10.1111/j.1365-2095.2006.00431.x
[9] C. Y. Cho and D. P. Bureau, “A Review of Diet Formulation Strategies and Feeding Systems to Reduce Excretory and Feed Wastes in Aquaculture,” Aquaculture Research, Vol. 32, No. S1, 2001, pp. 349-360. doi:10.1046/j.1355-557x.2001.00027.x
[10] P. Jahan, T. Watanabe, S. Satoh and V. Kiron, “Reduction in Elemental Waste Loading from Commercial Carp Feeds by Manipulating the Dietary Phosphorus Levels,” Fisheries Sciences, Vol. 69, No. 1, 2003, pp. 58-65. doi:10.1046/j.1444-2906.2003.00588.x
[11] V. Leonard, C. Breyne, J.-C. Micha and Y. Larondelle, “Digestibility and Transit Time of Azolla filiculoides Lamarck in Oreochromis aureus (Steindachner),” Aquaculture Research, Vol. 29, 1998, pp. 159-165. doi:10.1111/j.1365-2109.1998.tb01120.x
[12] E. A. Fasakin, A. M. Balogun and O. A. Fagbenro, “Evaluation of Sun-Dried Water Fern, Azolla africana, and Duckweed, Spirodela polyrrhiza, in Practical Diets for Nile Tilapia, Oreochromis niloticus, Fingerlings,” Journal of Applied Aquaculture, Vol. 11, No. 4, 2001, pp. 83-92. doi:10.1300/J028v11n04_09
[13] E. D. Fiogbé, J.-C. Micha and C. Van Hove, “Use of a Natural Aquatic Fern, Azolla microphylla, as a Main Component in Food for Omnivorous-Phytoplanktonophagous Tilapia, Oreochromis niloticus L.,” Journal of Applied Ichthyology, Vol. 20, No. 6, 2004, pp. 517-520. doi:10.1111/j.1439-0426.2004.00562.x
[14] Y. Abou, E. D. Fiogbé and J.-C. Micha, “A Preliminary Assessment of Growth and Production of Nile Tilapia, Oreochromis niloticus L., Fed Azolla-Based-Diets in Earthen Ponds,” Journal of Applied Aquaculture, Vol. 19, No. 4, 2007, pp. 55-69. doi:10.1300/J028v19n04_03
[15] Y. Abou, E. D. Fiogbé and J.-C. Micha, “Effects of Stocking Density on Growth, Yield and Profitability of Farming Nile Tilapia, Oreochromis niloticus L., Fed Azolla Diet, in Earthen Ponds,” Aquaculture Research, Vol. 38, No. 6, 2007, pp. 595-604. doi:10.1111/j.1365-2109.2007.01700.x
[16] V. Leonard, “Use of an Aquatic Fern (Azolla filiculoides) in Two Species of Tropical Fish (Oreochromis niloticus and Tilapia rendalli),” Doctoral Dissertation, Catholic University of Louvain, Louvain-la-Neuve, 1997.
[17] F. Carrapi?o, G. Teixeira and M. Adélia Diniz, “Azolla as a Biofertilizer in Africa. A Challenge for the Future,” Revista de Ciêcias Agrárias, Vol. 23, 2000, pp. 120-138.
[18] A. G. J. Tacon, “Standard Methods for the Nutrition and Feeding of Farmed Fish and shrimp,” Argent Laboratories Press, Washington DC, 1990.
[19] Ch. Mélard, “Bases Biologiques de l’élevage Intensif du Tilapia du Nil O. niloticus,” Cahiers d’Ethologie Appliquée, Vol. 5, No. 3, 1986, pp. 1-224.
[20] AOAC, “Official Methods of Analysis,” 15th Edition, Association of Official Analytical Chemists, Arlington, 1990.
[21] A. Gross and C. E. Boyd, “A Digestion Procedure for the Simultaneous Determination of Total Nitrogen and Total Phosphorus in Pond Water,” Journal of the World Aquaculture Society, Vol. 29, No. 3, 1998, pp. 300-303. doi:10.1111/j.1749-7345.1998.tb00650.x
[22] C. Y. Cho, J. D. Hynes, K. R. Wood and H. K. Yoshida, “Development of High-Nutrient-Dense, Low-Pollution Diets and Prediction of Aquaculture Waste Using Biological Approaches,” Aquaculture, Vol. 124, No. 1-4, 1994, pp. 293-305. doi:10.1016/0044-8486(94)90403-0
[23] H. O. Hartley, “Smallest Composite Designs for Quadratic Response Surface,” Biometrics, Vol. 15, No. 4, 1959, pp. 611-624. doi:10.2307/2527658
[24] D. B. Duncan, “Multiple Range and Multiple F-Tests,” Biometrics, Vol. 11, No. 1, 1955, pp. 1-42. doi:10.2307/3001478
[25] N. C. Sanginga and C. Van Hove, “Amino Acids of Azolla as Affected by Strains and Population Density,” Plant and Soil, Vol. 117, No. 2, 1989, pp. 263-267. doi:10.1007/BF02220720
[26] E. W. Becker, “Nutritional Properties of Microalgae: Potential and Constrains,” In A. Richmond, Ed., Handbook of Microalgae Mass Culture, CRC Press, Boca Raton, 1986.
[27] J.-C. Micha and V. Leonard, “Digestibility of the Aquatic Fern Azolla filiculoides Lamarck in Two Species of Tilapia: The Phytoplanktonophagous Oreochromis niloticus (L.) and the Macrophytophagous Tilapia rendalli (Boulenger),” Bulletin des Séances de l’Académie Royale des Sciences d’Outre-Mer, Vol. 47, 2001, pp. 147-157.
[28] A. Hernández, S. Shuichi, V. Kiron and T. Watanabe, “Phosphorus Retention in Rainbow Trout Fed Diets with Low Fish Meal and Alternative Protein Ingredients,” Fisheries Sciences, Vol. 70, No. 4, 2004, pp. 580-586. doi:10.1111/j.1444-2906.2004.00844.x
[29] T. Watanabe, T. Takeuchi, A. Murakami and C. Ogino, “The Availability to Tilapia nilotica of Phosphorus in White Fish Meal,” Bulletin of the Japanese Society of Scientific Fisheries, Vol. 46, No. 7, 1980, pp. 897-899. doi:10.2331/suisan.46.897
[30] J. S. Haylor, M. C. M. Beveridge and K. Jauncey, “Phosphorus Nutrition of Juvenile Oreochromis niloticus,” In: R. S. V. Pullin, T. Bhukaswan, K. Tonguthai and J. L. Maclean, Eds., The Second International Symposium on Tilapia in Aquaculture, Bangkok and ICLARM, Manila, 1998.
[31] S. J. Kaushik, D. Covès, G. Dutto and D. Blanc, “Almost Total Replacement of Fish Meal by Plant Protein Sources in the Diet of a Marine Teleost, the European Seabass, Dicentrarchus labrax,” Aquaculture, Vol. 230, No. 1-4, 2004, pp. 391-404. doi:10.1016/S0044-8486(03)00422-8
[32] H. G. Ketola and B. F. Harland, “Influence of Phosphorus in Rainbow Trout Diets on Phosphorus Discharges in Effluent Water,” Transactions of the American Fisheries Society, Vol. 122, No. 6, 1993, pp. 1120-1126. doi:10.1577/1548-8659(1993)122<1120:IOPIRT>2.3.CO;2
[33] M. J. Phillips, M. C. M. Beveridge and L. G. Ross, “The Environmental Impact of Salmonid Cage Culture on Inland Fisheries: Present Status and Future Trends,” Journal of Fish Biology, Vol. 27, Suppl. A, 1985, pp. 123-127. doi:10.1111/j.1095-8649.1985.tb03236.x
[34] Y. Abou, E. D. Fiogbe, M. P. Aina, A. Buldgen and J.-C. Micha, “Evaluation of Nitrogen and Phosphorus Wastes Produced by Nile Tilapia (Oreochromis niloticus L.) Fed Azolla-Diets in Concrete Tanks,” International Journal of Biological and Chemical Sciences, Vol. 4, No. 1, 2010, pp. 42-50. doi:10.4314/ijbcs.v4i1.54229

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