Interferon-gamma ELISPOT for the screening and diagnosis of latent tuberculosis infection in healthy population of China

Abstract

The aim of this study was to analyze the combination of three kinds of in-house IFN-γ ELISPOT using peptide A53 and peptide mixtures (E6 + E7 and E6 + E7 + C14) with tuberculin skin test (TST) to detect latent TB infection (LTBI) in China. A total of 788 healthy people were recruited and analyzed by three kinds of IFN-g ELISPOT, 581 of them had TST results, of which 147 samples were also compared with the T- SPOT.TB test. The positive detection rates for T- SPOT.TB and three kinds of IFN-γ ELISPOT with A53, E6 + E7 and E6 + E7 + C14 were 14.28% (21/147), 29.43% (171/581), 23.24% (135/581) and 28.40% (165/581), respectively. These results were significantly lower than the positive TST results, which were positive in 82.99% (122/147) and 75.73% (440/ 581), respectively. The positive detection rates of three kinds of IFN-γ ELISPOT (31.60%, 26.65% and 32.11% in 788 cases, respectively) could better reflect over 40.00% of Mycobacterium tuberculosis (MTB) infection rate in China. Detection rates between contacts and non-contacts by three kinds of IFN-γ ELIS-POT were not significantly different (p > 0.05). It can be seen that the three kinds of in-house IFN-γ ELIS- POT might be used as a complementary tool of T- SPOT.TB for detecting LTBI in the healthy population of China.

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Peng, Y. , Yang, F. , Zhou, L. , Zhao, M. , Li, Y. , Huang, Y. , Wang, J. , Huang, L. , Xie, D. , Tu, Z. , Lin, W. , Liu, J. , Zhong, Q. and Lai, X. (2012) Interferon-gamma ELISPOT for the screening and diagnosis of latent tuberculosis infection in healthy population of China. Advances in Bioscience and Biotechnology, 3, 259-268. doi: 10.4236/abb.2012.33036.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] World Health Organization (2007) WHO fact sheet. http://www.who.int/mediacentre/factsheets /fs104/en/
[2] Jasmer, R.M., Nahid, P. and Hopewell, P.C. (2002) Clinical practice. Latent tuberculosis infection. The New England Journal of Medicine, 347, 1860-1866. doi:10.1056/NEJMcp021045
[3] Huebner, R.E., Schein, M.F. and Jr. Bass, J.B. (1993) The tuberculin skin test. Clinical Infectious Diseases, 17, 968- 975. doi:10.1093/clinids/17.6.968
[4] Karlsson, A.C., Martin, J.N., Younger, S.R., Bredt, B.M., Epling, L., Ronquillo, R., Varma, A., Deeks, S.G., Mc Cune, J.M., Nixon, D.F. and Sinclair, E. (2003) Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells. Journal of Immunological Methods, 283, 141-153. doi:10.1016/j.jim.2003.09.001
[5] Tassignon, J., Burny, W., Dahmani, S., Zhou, L., Stor- deur, P., Byl, B. and De Groote, D. (2005) Monitoring of cellular responses after vaccination against tetanus toxoid: Comparison of the measurement of IFN-gamma production by ELISA, ELISPOT, flow cytometry and real-time PCR. Journal of Immunological Methods, 305, 188-198. doi:10.1016/j.jim.2005.07.014
[6] Menzies, D., Pai, M. and Comstock, G. (2007) Meta- analysis: New tests for the diagnosis of latent tuberculosis infection: Areas of uncertainty and recommendations for research. Annals of Internal Medicine, 146, 340-354.
[7] Hill, P.C., Jackson-Sillah, D.J., Fox, A., Brookes, R.H., de Jong, B.C., Lugos, M.D., Adetifa, I.M., Donkor, S.A., Aiken, A.M., Howie, S.R., Corrah, T., McAdam, K.P. and Adegbola, R.A. (2008) Incidence of tuberculosis and the predictive value of ELISPOT and Mantoux tests in Gambian case contacts. PloS One, 3, e1379. doi:10.1371/journal.pone.0001379
[8] Dheda, K., Smit, R.Z., Badri, M. and Pai, M. (2009) T- cell interferon-gamma release assays for the rapid immunodiagnosis of tuberculosis: Clinical utility in high burden vs low-burden settings. Current Opinion in Pulmonary Medicine, 15, 188-200. doi:10.1097/MCP.0b013e32832a0adc
[9] Casas, I., Latorre, I., Esteve, M., Ruiz-Manzano, J., Rodriguez, D., Prat, C., García-Olivé, I., Lacoma, A., Ausina, V. and Domínguez, J. (2009) Evaluation of in- terferon-gamma release assays in the diagnosis of recent tuberculosis infection in health care workers. PloS One, 4, e6686. doi:10.1371/journal.pone.0006686
[10] Wilkinson, K.A., Kon, O.M., Newton, S.M., Meintjes, G., Davidson, R.N., Pasvol, G. and Wilkinson, R.J. (2006) Effect of treatment of latent tuberculosis infection on the T cell response to Mycobacterium tuberculosis antigens. Journal of Infectious Diseases, 193, 354-359. doi:10.1086/499311
[11] Chee, C.B., KhinMar, K.W., Gan, S.H., Barkham, T.M., Pushparani, M. and Wang, Y.T. (2007) Latent tuberculosis infection treatment and T-cell responses to Mycobacterium tuberculosis-specific antigens. American Journal of Respiratory and Critical Care Medicine, 175, 282-287. doi:10.1164/rccm.200608-1109OC
[12] Sokal, J.E. (1975) Editorial: Measurement of delayed skintest responses. The New England Journal of Medicine, 293, 501-502. doi:10.1056/NEJM197509042931013
[13] American Thoracic Society and the Centers for Disease Control and Prevention (2000) Diagnostic standards and classification of tuberculosis in adults and children. American Journal of Respiratory and Critical Care Medicine, 161, 1376-1395.
[14] Li, Y., Zhu, Y., Zhou, L., Fang, Y., Huang, L., Ren, L., Peng, Y., Li, Y., Yang, F., Xie, D., Tang, W., Zhang, N., Zhong, Q. and Lai, X. (2011) Use of HLA-DR*08032/E7 and HLA-DR*0818/E7 tetramers in tracking of epitope-specific CD4+ T cells in active and convalescent tubercu- losis patients compared with control donors. Immunobiology, 216, 947-960. doi:10.1016/j.imbio.2011.01.003
[15] Ewer, K., Deeks, J., Alvarez, L., Bryant, G., Waller, S., Andersen, P., Monk, P. and Lalvani, A. (2003) Comparison of T-cell-based assay with tuberculin skin test for di- agnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Lancet, 361, 1168-1173. doi:10.1016/S0140-6736(03)12950-9
[16] Hill, P.C., Brookes, R.H., Fox, A., Fielding, K., Jeffries, D.J., Jackson-Sillah, D., Lugos, M.D., Owiafe, P.K., Donkor, S.A., Hammond, A.S., Out, J.K., Corrah, T., Adegbola, R.A. and McAdam, K.P. (2004) Large-scale evaluation of enzyme-linked immunospot assay and skin test for diagnosis of Mycobacterium tuberculosis infection against a gradient of exposure in the Gambia. Clinical Infectious Diseases, 38, 966-973. doi:10.1086/382362
[17] Ferrara, G., Losi, M., D’Amico, R., Roversi, P., Piro, R., Meacci, M., Meccugni, B., Dori, I.M., Andreani, A., Bergamini, B.M., Mussini, C., Rumpianesi, F., Fabbri, L.M. and Richeldi, L. (2006) Use in routine clinical practice of two commercial blood tests for diagnosis of infection with Mycobacterium tuberculosis: A prospective study. Lancet, 367, 1328-1334. doi:10.1016/S0140-6736(06)68579-6
[18] Jackson-Sillah, D., Hill, P.C., Fox, A., Brookes, R.H., Donkor, S.A., Lugos, M.D., Howie, S.R., Fielding, K.R., Jallow, A., Lienhardt, C., Corrah, T., Adegbola, R.A. and McAdam, K.P. (2007) Screening for tuberculosis among 2381 household contacts of sputum-smearpositive cases in the Gambia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 101, 594-601. doi:10.1016/j.trstmh.2007.02.001
[19] Guwatudde, D., Nakakeeto, M., Jones-Lopez, E.C., Ma- ganda, A., Chiunda, A., Mugerwa, R.D., Ellner, J.J., Bukenya, G. and Whalen, C.C. (2003) Tuberculosis in household con-tacts of infectious cases in Kampala, Uganda. American Journal of Epidemiology, 158, 887-898. doi:10.1093/aje/kwg227
[20] Egsmose, T., Ang’awa, J.O. and Poti, S.J. (1965) The use of isoniazid among house-hold contacts of open cases of pulmonary tuberculosis. Bulletin of the World Health Organ, 33, 419-433.
[21] Puffer, R.R., Zeidberg, L.D., Dillon, A., Gass, R.S. and Hutcheson, R.H. (1952) Tuberculosis attack and death rates of household associates; the influence of age, sex, race, and relationship. American Review of Tuberculosis, 65, 111-127.
[22] Ferebee, S.H. and Mount, F.W. (1962) Tuberculosis morbidity in a controlled trial of the prophylactic use of isoniazid among household contacts. The American Review of Respiratory Disease, 85, 490-510.
[23] Nienhaus, A., Schablon, A. and Diel, R. (2008) Inter- feron-gamma release assay for the diagnosis of latent TB infection-analysis of discordant results, when compared to the tuberculin skin test. PLoS One, 3, e2665. doi:10.1371/journal.pone.0002665
[24] Piana, F., Ruffo Codecasa, L., Baldan, R., Miotto, P., Ferrarese, M. and Cirillo, D.M. (2007) Use of T-SPOT. TB in latent tuber-culosis infection diagnosis in general and immunosup-pressed populations. New Microbiologica, 30, 286-290.
[25] Pai, M., Zwerling, A. and Menzies, D. (2008) Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: An update. Annals of Internal Medicine, 149, 177-184.

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