Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

DOI: 10.4236/abb.2012.33035   PDF   HTML     3,646 Downloads   6,739 Views   Citations


Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

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Yam-Puc, A. , Avilés-Berzunza, E. , Chan-Bacab, M. , Peña-Rodríguez, L. and Godoy-Hernández, G. (2012) Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.. Advances in Bioscience and Biotechnology, 3, 256-258. doi: 10.4236/abb.2012.33035.

Conflicts of Interest

The authors declare no conflicts of interest.


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