Anti-Microbial and Physico-Chemical Properties of Processed and Raw Honeys of Mauritius


Among the panoply of food products used in treatment of various ailments, honey has been used since prehistoric times as an effective antimicrobial therapy. Being first of its kind, honeys with specific botanical origin from Mauritius were examined. In this study, three commercially processed-enhanced v/s three unprocessed honeys were evaluated for possible antimicrobial activities against clinical bacterial (Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Pseudomonas aeroguinosa (ATCC 27853)) and fungal (Aspergillus niger (ATCC 16404) and Candida albicans (ATCC 10231)) isolates in vitro. Research findings showed that the respective physicochemical parameters of the samples ranged from; pH: 3.28 - 4.67, H2O: 17.63% - 24.87%, reducing sugar 42.95 - 60.31 g/100g, density 1.11 - 1.55 g/ml and viscosity: 1.85 - 26.24 Pa·s at 20°C. Physicochemical parameters of different honeys were observed to vary significantly with respect to their floral origin (P < 0.05). After initial screening of the honeys tested at 100% (without dilution), minimum inhibitory concentration were established at three graded concentrations of 50%, 25% and 12.5% respectively. Promising results were observed against all clinical isolates evaluated; with inhibition zones ranging from 6 ± 0.00 to 20 ± 0.01 mm for undiluted honey samples compared to standard ampicillin from 14 ± 1.00 to 17 ± 3.06. The fungus used was found to be more resistant to the honeys than the bacteria. However, both processed and raw honeys were observed to possess highly significant antibacterial and antifungal properties (P < 0.05). The honey samples used in this study can be considered as valuable food products from their antimicrobial properties’ point of view and can have clinical potential.

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M. Shafiiq Kinoo, M. Fawzi Mahomoodally and D. Puchooa, "Anti-Microbial and Physico-Chemical Properties of Processed and Raw Honeys of Mauritius," Advances in Infectious Diseases, Vol. 2 No. 2, 2012, pp. 25-36. doi: 10.4236/aid.2012.22005.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] World Health Organisation, “Containing Antimicrobial Resistance: A Renewed Effort,” Bulletin of the World Health Organization, Vol. 88, No. 12, 2010, pp. 877-953.
[2] E. R. Choffnes, D.A. Relman and A. Mack, “Antibiotic Resistance: Implications for Global Health and Novel Intervention Strategies,” Workshop Summary, National Aca -demies Sciences, Washington DC, 2010.
[3] World Health Organisation, “Traditional Medcines,” Media Centre, No. 134, 2008.
[4] D. P. Mohapatra, V. Thakur and S. K. Brarl, “Antibacterial Efficacy of Raw and Processed Honey,” Biotechnology Research International, Vol. 2011, 2011, pp. 1-6. doi:10.4061/2011/917505
[5] R. Jenkins, N. Burton and R. Cooper, “Effect of Manuka Honey on the Expression of Universal Stress Protein A in Meticillin-Resistant Staphylococcus aureus,” International Journal of Antimicrobial Agents, Vol. 37, No. 4, 2011, pp. 373-376. doi:10.1016/j.ijantimicag.2010.11.036
[6] R. T. Cursons, “The Post-Antibiotic Effect of Manuka Honey on Gastrointestinal Pathogens,” International Journal of antimicrobial Agents, Vol. 36, No. 5, 2010, pp. 467-482. doi:10.1016/j.ijantimicag.2010.06.046
[7] J. Irish, D. E. Carter, S. E. Blair and T.A. Heard, “Antimicrobial Activity of Honey from the Australian Stingless Bee Trigona carbonaria,” Journal of Antimicrobial Agents, Vol. 32, No. 1, 2008, pp. 89-98. doi:10.1016/j.ijantimicag.2008.02.012
[8] S. Kumari, K. Harjai and S. Chhibber, “Topical Treatment of Klebsiella Pneumoniae B5055 Induced Burn Wound Infection in Mice Using Natural Products,” Journal of Infection in Developing Countries, Vol. 4, No. 6, 2010, pp. 367-377.
[9] S. S. Zaid, S. A. Sulaiman, K. N. M. Sirajudeen and N. H. Ortman, “The Effects of Tualang Honey on Female Reproductive Organs, Tibia Tone and Hormonal Profile in Ovariectomised Rats-Animal Model for Menopose,” BMC Complementary and Alternative Medicine, Vol. 10, No. 82, 2010. doi:10.1186/1472-6882-10-82
[10] P. L. Conway, R. Stem and L. Tran, “The Value-Adding Potential of Prebiotic Components of Australian Honey,” Rural Industries Research and Development Corporation, Barton, Australia, 2010.
[11] A. Giorgi, M. Madeo, J. Baumgartner and G. C. Lozzia, “The Relationships between Phenolic Content, Pollen Diversity, Physicochemical Information and Radical Scavenging Activity in Honey,” Mololecules, Vol. 16, No. 1, 2011, pp. 336-347. doi:10.3390/molecules16010336
[12] A. N. Fauzi, M. N. Norazmi and N. S. Yaacob, “Tualang Honey Induces Apoptosis and Disrupts the Mitochondrial Membrane Potential of Human Breast and Cervical Cancer Cell Lines,” Food and Chemical Toxicology, Vol. 49, No. 4, 2011, pp. 871-878. doi:10.1016/j.fct.2010.12.010
[13] M. E. Conti, J. Stripeikis, L. Campanella, D. Cucina and M. B. Tudino, “Characterization of Italian Honeys (Marche Region) on the Basis of Their Mineral Content and Some Typical Quality Parameters,” Chemistry Central Journal, Vol. 1, No. 14, 2007, p. 14. doi:10.1186/1752-153X-1-14
[14] O. Celechovska and L. Vorlova, “Groups of HoneyPhysico-Chemical Properties and Heavy Metals,” Acta Veterinaria Brno, Vol. 70, No. 1, 2001, pp. 91-95. doi:10.2754/avb200170010091
[15] C. A. C, “Codex Standard for Honey,” FAO Agricultural Services Bulletin, Value Added Products from Beekeeping, Agriculture and Consumer Protection Department, Rome, 1996. e30.htm
[16] Central Statistics Office, “Statistics on Food and Agricultural Imports Trade Data for Agricultural Products,” Agricultural Information Division, Ministry of Agro Industry and Food Security, Mauritius, 2009.
[17] R. Belmin, “Intérêt, Faisabilité, et Modalités de Mise en Place d’Une Indication Géographique pour le Miel de Rodrigues,” Ecole Nationale Supérieure d’Agronomie et des Industries Alimentaires, Ile de la Réunion, 2010.
[18] K. Helrich, “Official Methods of Analysis of AOAC INTERNATIONAL,” AOAC INTERNATIONAL, Gaithersburg, 1990.
[19] H. Tavakolipur and A. K. Ashtari, “Rheology of Selected Persian Honeys,” International Journal of Food Engineering, Vol. 6, No. 3, 2010. doi:10.2202/1556-3758.1799
[20] A. B. Gidamis, B. E. Chove, N. B. Shayo, S. A. Nnko and N. T. Bangu, “Quality Evaluation of Honey Harvested from Selected Areas in Tanzania with Special Emphasis on Hydroxymethyl Furfural (HMF) Levels,” Plant Foods for Human Nutrition, Vol. 59, No. 3, 2004, pp. 129-132. doi:10.1007/s11130-004-0020-7
[21] J. S. Kumar and M. Mandal, “Rheology and Thermal Properties of Marketed Indian Honey,” Nutrition & Food Science, Vol. 39, No. 2, 2009, pp. 111-117. doi:10.1108/00346650910943217
[22] United State Department of Agriculture, “United States Standards for Grades of Extracted Honey,” United State Department of Agriculture, Washington DC, 1985.
[23] R. S. Kirk and R. Sawyer, “Pearson’s Composition and Analysis of Foods,” 9th Edition, Longman Scientific and Technical, Essex, 1991.
[24] International Standard Organisation, “Fruit and Vegetable Products—Determination of Soluble Solids—Refractometric Method,” 2nd Edition, International Organisation for Standardisation, Geneva, 1998.
[25] O. Sherlock, A. Dolan, R. Athman, A. Power, G.Gethin , S. Cowman and H. Humphreys, “Comparison of the Antimicrobial Activity of Ulmo Honey from Chile and Manuka Honey Against Methicillin-Resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeroginosa,” BMC Complementary and Alternative Medicine, Vol. 10, No. 47, 2010. doi:10.1186/1472-6882-10-47
[26] O. E. Agbagwa and N. F. Peterside, “Effect of Raw Commercial Honeys from Nigeria on Selected Pathogenic Bacteria,” African journal of Microbiology Research, Vol. 4, No. 16, 2010, pp. 1801-1803.
[27] A. A. Ansari and C. Alexander, “Effect of Natural Honey (Produced by African sculata in Guyana) Against Bacteria (Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus) and Fungus (Candida albicans),” World Dairy & Food Science, Vol. 4, No. 1, 2009, pp. 73-77.
[28] N. J. Basson and S. R. Grobler, “Antimicrobial Activity of two South African Honeys Produced from Indigenous Leucospermum cordifolium and Erica Species on Selected Micro-Organisms,” BMC Complementary and Alternative Medicine, Vol. 8, No. 41, 2008. doi:10.1186/1472-6882-8-41
[29] M. F. Mahomoodally, A. Gurib-fakim and A. H. Subratty, “Screening for Alternative Antibiotics: An Investigation into Antimicrobial Activities of Medical Food Plants of Mauritius,” Journal of Food Science, Vol. 75, No. 3, 2010, pp. 173-177. doi:10.1111/j.1750-3841.2010.01555.x
[31] D. Gomez-Diaz, J. M. Navaza and L. C. Quintans-Riveiro, “Rheological Behaviour of Galician Honeys,” The Journal of European Food Research and Technology, Vol. 222, No. 3-4, 2006, pp. 439-442. doi:10.1007/s00217-005-0120-0
[32] S. Saxena, S. Gautam and A. Sharma, “Physical, Biochemical and Antioxidant Properties of some Indian honeys,” Journal of Food Chemistry, Vol. 118, No. 2, 2010, pp. 391-397. doi:10.1016/j.foodchem.2009.05.001
[33] M. Mohamed, K. N. S. Sirajudeen, M. Swamy, S. Y. Nik and A. S. Siti, “Studies on the Antioxidant Properties of Tualang Honey of Malaysia,” African Journal of Traditional, Complementary and Alternative Medicines, Vol. 7, No. 1, 2010, pp. 59-63. doi:10.4314/ajtcam.v7i1.57256
[34] H. D. Belitz, W. Grosch and P. Schieberle, “Food Chemistry,” 4th Edition, Springer, Heidelberg, 2009.
[35] J. Raloff, “The Color of Honey,” Science News, Vol. 154, No. 11, 1998, pp. 170-172. doi:10.2307/4010759
[36] M. S. Finola, M. C. Lasagno and J. M. Marioli, “Microbiological and Chemical Characterisation of Honeys from Central Argentina,” Food Chemistry, Vol. 100, No. 4, 2008, pp. 1649-1653. doi:10.1016/j.foodchem.2005.12.046
[37] E. F. Phillips, “Definitions of Honey Colour Grades,” Journal of Agricultural Research, Vol. 45, No. 12, 1932, pp. 757-770.
[38] H. Clément, “Guide des Miels: 40 Miels à Découvrir,” Rustica Editions, Paris, 2002.
[39] S. Bogdanov, “Books of Honey, Physical Properties of Honey,” Bee Product Science, Zhejiang, 2011.
[40] N. S. Al-Waili, M. Akmal, F. S. Al-Waili, K. Y. Saloom and A. Ali, “The Antimicrobial Potential of Honey from United Arab Emirates on Some Microbial Isolates,” Medical Science Monitor, Vol. 11, No. 12, 2005, pp. 433-438.
[41] M. O. Adenekan, N. A. Amusa, A. O. Lawal and V. E. Okpeze, “Physico-Chemical and Microbiological Properties of Honey Samples Obtained from Ibadan,” Journal of Microbiology and Antimicrobials, Vol. 2, No. 8, 2010, pp. 100-104.
[42] P. E. Lusby, A. L. Coombes and J. M. Wilkinson, “Bactericidal Activity of Different Honeys against Pathogenic Bacteria,” Archives of Medical Research, Vol. 36, No. 5, 2005, pp. 464-467. doi:10.1016/j.arcmed.2005.03.038
[43] M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernandez-Lopez and J. A. Perez-Alvarez, “A Review: Functional Properties of Honey, Propolis, and Royal Jelly,” Journal of Food Science, Vol. 73, No. 9, 2008, pp. R117-R124. doi:10.1111/j.1750-3841.2008.00966.x
[44] B. S. SHAM, “Antioxidant and Flavonoid Profiles of Mauritian Honeys,” BSc (Hons) Biology Dissertation, University of Mauritius, Reduit, 2010.
[45] E. Halawani and M. Shohayeb, “Evaluation of the AntiBacterial Potential of Selected Honeys Available in Saudi Markets on Some Infectious and Food Spoilage Bacteria,” International Journal of Microbiological Research, Vol. 1, No. 2, 2010, pp. 45-49.
[46] B. Ray, “Fundamental Food Microbiology,” 3rd Edition, CRC Press, Boca Raton, 2005.
[47] M. S. Hassanein, M. H. Gebreel, and A. A. R. Hassan, “Honey Compared with Some Antibiotics against Bacteria Isolated from Burn-Wound Infections of Patients in Ain Shams University Hospital,” Journal of American Science, Vol. 6, No. 10, 2010, pp. 301-320.
[48] P. H. Kwakman, A. A. Te Velde, D. Speijer, C. M. Vandenbroucke-Grauls and S. A. Zaat, “How Honey Kills Bacteria,” Journal of Federation of American Societies for Experimental Biology, Vol. 25, No. 4, 2010, pp. 1145-1155.

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