Share This Article:

The Difference in Calcium Levels in Aspergillus nidulans Grown on Glucose or Pectin

Abstract Full-Text HTML XML Download Download as PDF (Size:379KB) PP. 117-121
DOI: 10.4236/aim.2012.22016    5,660 Downloads   8,780 Views   Citations

ABSTRACT

Understanding the growth regulatory mechanisms in filamentous fungi is very important for the production of medicines for antifungal therapies. It is well established that Ca2+ gradient is essential for hyphal growth and that one mechanism responsible for the Ca2+ cellular concentration starts with the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by receptor-regulated forms of phosphoinositide-specific phospholipase C (PI-PLC). In the present study the levels of calcium in Aspergillus nidulans wild type (A26) and plcA-deficient mutant (AP27) growing in a carbon source readily assimilated, as glucose or pectin a non-readily assimilated carbon source was investigated. Intracellular calcium levels in A26 were higher in the presence of glucose than in pectin, but lower in AP27 independently of the carbon source and in AP27 the vesicular calcium distribution occurred mainly at the apex of the hyphae. Delay in nuclear division was also observed if A26 and AP27 were grown in pectin presence when compared with growth in glucose. For the first time, it is demonstrated that the levels of intracellular Ca2+ were higher when A. nidulans was growing in glucose than in a non readily assimilated carbon source as pectin. Further, it also showed that the plcA gene, although not essential, may be responsible for high-molecular weight carbon source recongnation, for the intracellular Ca2+ levels maintenance and consequently by the nuclear division in A. nidulans.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

J. Aparecida Rafael and S. Said, "The Difference in Calcium Levels in Aspergillus nidulans Grown on Glucose or Pectin," Advances in Microbiology, Vol. 2 No. 2, 2012, pp. 117-121. doi: 10.4236/aim.2012.22016.

References

[1] P. R. Kraus, C. B. Nichols and J. Heitman, “Calcium- and Calcineurin-Independent Roles for Calmodulin in Cryptococcus neoformans Morphogenesis and High-Temperature Growth,” Eukariotic Cell, Vol. 4, No. 6, 2005, pp. 1079-1087. doi:10.1128/EC.4.6.1079-1087.2005
[2] O. Gavric, D. B. Santos and A. Griffiths, “Mutation and Divergence of the Phospholipase C Gene in Neurospora crassa,” Fungal Genetics and Biology, Vol. 44, No. 4, 2007, pp. 242-249. doi:10.1016/j.fgb.2006.09.010
[3] T. Sone and A. J. Griffiths, “The Frost Gene of Neurospora crassa Is a Homolog of Yeast cdc1 and Affects Hyphal Branching via Manganese Homeostasis,” Fungal Genetics and Biology, Vol. 28, No. 3, 1999, pp. 227-237. doi:10.1006/fgbi.1999.1169
[4] M. J. Berridge, “Inositol Triphosphate and Calcium Signaling,” Nature, Vol. 361, No. 6410, 1993, pp. 315-325. doi:10.1038/361315a0
[5] Y. Nishizuka, “Intracellular Signaling by Hydrolysis of Phospholipids and Activation of Protein Kinase C,” Science, Vol. 258, No. 5082, 1992, pp. 607-614. doi:10.1126/science.1411571
[6] R. H. Michell, “Inositol Lipids in Cellular Signaling Mechanisms,” Trends in Biochemical Sciences, Vol. 17, No. 8, 1992, pp. 274-276. doi:10.1016/0968-0004(92)90433-A
[7] M. A. S. C. Chellegatti, P. D. Yuvamoto and S. Said, “Role of Phospholipase C and Protein Kinase C in Aspergillus nidulans during Growth on Pectin or Glucose: Effects on Germination and Duplication Cycle,” Folia Microbiologica, Vol. 55, No. 3, 2010, pp. 228-232. doi:10.1007/s12223-010-0033-6
[8] A. P. F. C. Vanzela, S, Said and R. A. Prade, “Phosphatidyl Inositol Phospholipase C Mediates Carbon Sensing and Vegetative Nuclear Duplication Rates in Aspergillus nidulans,” Canadia Journal of Microbiology, Vol. 57, No. 7, 2011, pp. 611-616. doi:10.1139/w11-034
[9] A. P. F. C. Vanzela and S. Said, “Evidence for Carbon Source Regulated Protein Kinase A and Protein Kinase C Signaling in the Duplication Cycle, Polarization and Septum Formation in Aspergillus nidulans,” Microbiologycal Research, Vol. 157, No. 3, 2002, pp. 239-247 doi:10.1078/0944-5013-00156
[10] C. Y. Kawano and S. Said, “Hyperbranching Induced by Cold-Shock or Snow-Flake Mutation in Neurospora crassa Is Prevented by Adition of Exogenous Calcium,” Journal of Basic Microbiology, Vol. 45, No. 3, 2005, pp. 199-206. doi:10.1002/jobm.200410496
[11] H. Prokisch, O. Yarden, M. Dieminger, M. Tropschug and I. B. Barthelmess, “Impairment of Calcineurin Function in Neurospora crassa Reveals Its Essential Role in Hyphal Growth, Morphology and Maintenance of the Apical Ca2+ Gradient,” Molecular and General Genetics, Vol. 256, No. 2, 1997, pp. 104-114. doi:10.1007/s004380050551
[12] L. B. Silverman-Gavrila, R. R. Lew, “An IP3-Activated Ca2+ Channel Regulates Fungal Tip Growth,” Journal of Cell Science, Vol. 115, No. 24, 2002, pp. 5013-5025. doi:10.1242/jcs.00180
[13] C. M. Calvert and D. Sanders, “Inositol Triphosphate-Dependent and Independent Ca2+ Mobilization Pathways at the Vacuolar Membrane of Candida albicans,” The Journal of Biological Chemistry, Vol. 70, 1995, pp. 7272-7280.
[14] P. J. M. Belde, J. H. Vossen, G. W. J. Borst-Pauwels and A. P. R. Theuvenet, “Inositol 1,4,5-Triphosphate Releases Ca2+ from Vacuolar Membrane Vesicles of Saccharomyces cerevisiae,” FEBS Letters, Vol. 23, No. 1-2, 1993, pp. 113-118. doi:10.1016/0014-5793(93)81460-H
[15] G. Cornelius, G. Gebauer and D. Techel, “Inositol Triphosphate Induces Calcium Release from Neurospora crassa Vacuoles,” Biochemical and Biophysical Research Communications, Vol. 162, No. 2, 1989, pp. 852-856. doi:10.1016/0006-291X(89)92388-7
[16] C. Muller, A. B. Spohr and J. Nielsen “Role of Substrate Concentration in Mitosis and Hyphal Extension of Aspergillus”, Biotechnology and Bioengineering, Vol. 67, No. 4, 2000, pp. 390-397. doi:10.1002/(SICI)1097-0290

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.