A Study of Scanning Electron Microscope of Vancomycin Resistant Enterococcus faecalis from Clinical Isolates

Ajay Kumar Oli, Raju Sungar, Nagaveni Shivshetty, Rajeshwari Hosamani, Kelmani Chandrakanth Revansiddappa

Department of Biotechnology, Gulbarga University, Gulbarga, India.
ELLA Foundation, Hyderabad, India.

DOI: 10.4236/aim.2012.22012                

Abstract

Vancomycin-resistant Enterococcus faecalis pose an emerging health risk, but little is known about the precise epidemiology for vancomycin resistance. The glycopeptide resistant was studied using different techniques such as broth macrodilution, agar dilution combined with agar diffusion, morphology cell changes by scanning electron microscopy. Eight VREF isolated from different clinical samples were used. Results showed low level and high level resistant to vancomycin antibiotic at concentration of 64 to 128 μg/ml, but antibacterial activity was reduced to 256 μg/ml, the SEM revaled increased in the cell size with the antibiotic compared to control and standard culture. The technique constitutes simple method for the detection of organism.

Keywords

VREF; SEM; Vancomycin

Conflicts of Interest

The authors declare no conflicts of interest.

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