Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems
Natalia A. Karnaukhova, Larisa A. Sergievich, Valery N. Karnaukhov
DOI: 10.4236/ns.2010.25054   PDF   HTML     5,668 Downloads   10,099 Views   Citations


Spectral luminescent analysis of single cells and cellular systems enable us to reveal the initial changes of intracellular metabolism that can followed by human diseases or failure in biocenosis. Two cytodiagnostic systems of de-vices and techniques have been developed: 1) Microspectrofluorimeters registering the fluo-rescent spectra of individual cells or intracellular organelles used for fundamental investigations of cell reactions and for discovering and studying new dimensionless fluorescent characteristic parameters reflecting the biochemical or physiological properties of the cells; 2) Double- and multi-wave microfluorimeters for rapid registration of fluorescent characteristic para- meters for many cells to obtain statistical information about cell population. These techni- ques are useful especially in medical and eco-logical investigations.

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Karnaukhova, N. , Sergievich, L. and Karnaukhov, V. (2010) Application of microspectral luminescent analysis to study the intracellular metabolism in single cells and cell systems. Natural Science, 2, 444-449. doi: 10.4236/ns.2010.25054.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Karnaukhov, V.N. (1978) Luminescent spectral analysis of cell. Nauka, Moscow.
[2] Karnaukhov, V.N. (2001) Spectral analysis in cell-level monitoring of environmental state. Nauka, Moscow.
[3] Karnaukhov, V.N. (1978) Luminescent analysis of cell. Nauka, Moscow. portal/e-library/ 00000048/00000048.htm
[4] Karnaukhov, V.N., Yashin, V.A., Karnaukhova, N.A., Kazantsev, A.P. and Karnaukhov, A.V. (1999) Double-wave microfluorimeter “Radical DMF-2”. Book of Abstracts II Congress of Biophysicists of Russia, 2, 594-595.
[5] Karnaukhova, N.A., Sergievich, L.A., Kuzhevskij, B.M. Sigaeva, E.A., Nechaev, O.Y., Karnaukhov, V.A. and Karnaukhov, V.N. (2007) A study of the correlation be-tween the functional activity of blood lymphocytes in different animals and intensity of neutrons near the earth surface. Biophysics, 52(4), 699-704.
[6] Karnaukhova, N.A. (1984) Luminescence parameters of blood nuclear cells in process of immune reaction in or-ganism. Biophysics, 29(2), 276-279.
[7] Karnaukhova, N.A. (1991) Changes in fluorescent spectra of acridine orange stained blood cells from patient suffering from lymphosarcoma and leukemias in the course of chemotherapy. Experimental Oncology, 13(1), 50-53.
[8] Gordon, R.Y., Bocharova, L.S., Kruman, I.I., Popov, V.I., Kazantsev, A.P., Khutzian, S.S. and Karnaukhov, V.N. (1997) Acridine orange as an indicator of ribosome state in cell. Cytometry, 29(3), 215-221.
[9] Karnaukhova, N.A., Sergiyevich, L.A., Aksenova, G.E. and Karnaukhov, V.N. (1999) Synthetic activity of rat blood lymphocytes under acute and continuous gamma irradiation-fluorescent microspectral study. Radiation and Environmental Biophysics, 38(1), 49-56.
[10] Karnaukhova, N.A., Lubet, P.Е., Katania, R., Karnaukhov, А.V., Sergievich, L.A. and Karnaukhov, V.N. (2003) Microspectral studies on neuroendocrine regulation of gametogenesis in mollusk. Biophysics, 48(5), 869-873.
[11] Karnaukhova, N.A., Sergiyevich, L.A. and Karnaukhov, V.N. (2008) Dinamics of ribosomal activity and protein production in peripheral blood lymphocytes during an immune response. Biophysics, 53(4), 632-637.
[12] Karnaukhova, N.A., Sergievich, L.A., Kvakina, E.B., Barsukova, L.P., Mar’yanovskaya, G.Y. and Kuz’-menko, T.S. (2000) Study into the changes in the functional state of the synthesis apparatus of blood lymphocytes under the action of weak low-frequency magnetic fields. Biophysics, 45(4), 697-703.
[13] Karnaukhova, N.A., Sergievich, L.A., Karnaukhov, V.A. and Karnaukhov, V.N. (2004) Changes in the synthetic activity of lymphocytes under the action of physical fac-tors related to Solar activity variations. Biophysics, 49(suppl.1), 552-559.
[14] Karnaukhov, V.N., Lebedev, O.E. and Pavlenko, V.K. (1976) About two mitochondrial pools in a stretch-receptor neuron. Tsitologia, 18(10), 1189-1193.
[15] Rudenko, Y.N., Bigdai, E.V. and Samoilov, V.O. (2007) Kinetics of Са2+, NADH and oxidized flavoproteids in the frog olfactory living under the effect of odorants. Bi-ophysics, 52(1), 88-94.
[16] Thorell, B. (1981) Flow cytometric analysis of cellular endogenous fluorescence. Cytometry, 2(1), 39-43.
[17] Roshchina, V.V. (2003) Autofluorescence of plant se-creting cells as a biosensor and bioindicator reaction. Journal of Fluorescence, 13(5), 403-420.
[18] Karnaukhov, V.N., Martsenuk P.P. and Yashin, V.A. (1980) Luminescence spectral characteristics of physio-logical state of cells of blue-green algae. Fisiologia Rast, 27(1), 11-17.
[19] Karnaukhov, V.N. and Yashin, V.A. (2003) Spectral stu-dies on single cells of sea microplankton: History and prospects. Biophysics, 48(5), 940-949.

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