Genotoxic Potential of Shrimp Pastes (Belacan) Extracts Using Umu Test


This study was conducted to observe the genotoxic effects of aqueous, methanol, hexane and dichloromethane extracts of “belacan” (shrimp paste) taken from three local districts in Melaka, Malaysia (Kelemak, Batang Tiga & Pantai Puteri). The umu test which was used as the screening test was conducted with and without the presence of metabolic activation system. Without the presence of metabolic activation system, aqueous extracts from Kelemak showed mutagenicity activity at 5 mg/ml with IR (Induction Rate) = 1.52 ± 0.57 and the methanol extracts showed mutagenic activities at 0.625 mg/ml and 5 mg/ml, which the IR was the highest at 5 mg/ml (2.08 ± 0.09). On the other hand, samples from Batang Tiga, Melaka showed mutagenic effects at all five concentrations for the dichloromethane extract, with IR = 2.09 ± 0.64 as the highest value at 1.25 mg/ml. Methanol extracts also showed positive results at 1.25 mg/ml and 2.5 mg/ml with IR = 1.70 ± 0.33 and IR = 2.12 ± 0.51 respectively, and aqueous extract at 0.625 mg/ml with IR = 1.54 ± 0.48 and 5 mg/ml with IR = 1.74 ± 0.50. There was a significant difference of the mean values of IR between the four different types of “belacan” extracts from Batang Tiga (p < 0.05). All four “belacan” extracts from Pantai Puteri, Melaka did not show any mutagenic effect. With the presence of metabolic activation system, there was no mutagenic effect observed in all four extracts from the three districts. Further study to analyze the contents in the food samples should be done in the future to determine the possible contents in the food samples that might be responsible for the mutagenic activities.

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A. Ghazali, Y. Sipain, N. Rajab, S. Ling, N. Ramli, R. Abdullah, Z. Harun and F. Kamarulzaman, "Genotoxic Potential of Shrimp Pastes (Belacan) Extracts Using Umu Test," Food and Nutrition Sciences, Vol. 3 No. 4, 2012, pp. 522-525. doi: 10.4236/fns.2012.34073.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] B. T. Smith and G. C. Walker, “Mutagenesis and More: umuDC and the Escherichia Coli SOS Response,” Genetics, Vol. 148, No. 4, 1998, p. 1599.
[2] B. S. Gendeh, S. Murad, A. M. Razi, N. Abdullah, A. S. Mohamed and K. A. Kadir, “Skin Prick Test Reactivity to Foods in Adult Malaysians with Rhinitis,” Otolaryngology-Head and Neck Surgery, Vol. 122, No. 5, 2000, pp. 758-762. doi:10.1016/S0194-5998(00)70211-7
[3] F. Arshad, L.P.K., L. H. Yee, S. Nooriah, S. Suzana, A. T. Ruzita, M. S.Lingam and E. P. Tan, “A Retrospective Case-Control Study: The Association Between Dietary Intake and Cancer,” Department of Nutrition and Dietetics, UKM, National Cancer Council, 2005.
[4] IARC, “Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans,” IARC, Lyon, 1993.
[5] T. Kawabata, J. Uibu, H. Ohshima, M. Matsui, M. Hamano and H. Tokiwa, “Occurrence, Formation and Precursors of N-Nitroso Compounds in the Japanese Diet,” IARC Scientific Publications, Vol. 31, 1980, p. 481.
[6] A. R. Ghazali, N. F. Rajab, R. Sharif, T. A. Yaakob and F. Arshad, “The Genotoxicological Evaluation of Several Local Raw Foods Extracts on Chang Liver Cells by Single Cell Electrophoresis Assay,” Environmental Mutagen Research, Vol. 27, No. 3, 2005, pp. 165-170. doi:10.3123/jems.27.165
[7] M. Takahashi and R. Hasegawa, “Enhancing Effects of Dietary Salt on Both Initiation and Promotion Stages of Rat Gastric Carcinogenesis,” In: Y. Hayashi, et al., Eds., Diet, Nutrition and Cancer, Japan Science Society Press, Utrecht, 1986, pp. 169-182.
[8] R. K. Phukan, K. Narain, E. Zomawia, N. C. Hazarika and J. Mahanta, “Dietary Habits and Stomach Cancer in Mizoram, India,” Journal of Gastroenterology, Vol. 41, No. 5, 2006, pp. 418-424. doi:10.1007/s00535-006-1761-x
[9] M. Haidekker, T. Brady, D. Lichlyter and E. Theodorakis, “Effects of Solvent Polarity and Solvent Viscosity on the Fluorescent Properties of Molecular Rotors and Related Probes,” Bioorganic Chemistry, Vol. 33, No. 6, 2005, pp. 415-425. doi:10.1016/j.bioorg.2005.07.005
[10] K. Mortelmans and E. Zeiger, “The Ames Salmonella/Microsome Mutagenicity Assay,” Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 455, No. 1-2, 2000, pp. 29-60.
[11] B. Hamer, N. Bihari, G. Reifferscheid, R. K. Zahn, W. Müllerc and R. Batel, “Evaluation of the SOS/Umu-Test Post-Treatment Assay for the Detection of Genotoxic Activities of Pure Compounds and Complex Environmental Mixtures,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 466, No. 2, 2000, pp. 161-171. doi:10.1016/S1383-5718(00)00016-4
[12] K. Ozturk and M. Durusoy, “The Detection and Comparison of the Genotoxic Effects of Some Nitro Aromatic Compounds by the Umu and SOS Chromotest Systems,” Toxicology Letters, Vol. 108, No. 1, 1999, pp. 63-68. doi:10.1016/S0378-4274(99)00117-4
[13] L. Verschaeve and J. Van Staden, “Mutagenic and Antimutagenic Properties of Extracts from South African Traditional Medicinal Plants,” Journal of Ethnopharmacology, Vol. 119, No. 3, 2008, pp. 575-587. doi:10.1016/j.jep.2008.06.007
[14] R. Sharif, A. Ghazali, N. Rajab, H. Haron and F. Osman, “Toxicological Evaluation of Some Malaysian Locally Processed Raw Food Products,” Food and Chemical Toxicology, Vol. 46, No. 1, 2008, pp. 368-374. doi:10.1016/j.fct.2007.08.010
[15] T. Sumino, S. Kageyama, E. Endo, R. Chihara and K. Yamada, “Components and Bacterial Content of Belacan Produced in Malaysia,” Journal of Cookery Science of Japan, Vol. 36, No. 2, 2003, pp. 164-169.
[16] A. Cohen and F. Roe, “Evaluation of the Aetiological Role of Dietary Salt Exposure in Gastric and Other Cancers in Humans,” Food and Chemical Toxicology, Vol. 35, No. 2, 1997, pp. 271-293. doi:10.1016/S0278-6915(96)00114-7
[17] E. Hodgson and P. E. Levi, “A Textbook of Modern Toxicology,” Wiley Online Library, 2004. doi:10.1002/0471646776

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