Choice for host-specific high-adhesive Lactobacillus strains

Xueyan Lin, Zhonghua Wang, Zhongxiang Niu, Hongjie Liu, Yun Wang

.
College of Animal Science and Technology, Shandong Agricultural University, Tai’an, China.

DOI: 10.4236/abb.2012.32022                

Abstract

Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important observations regarding the adhesive ability between different Lactobacillus strains and three cell types: the adhesive interaction between the SDnB₇, SDnE₁ and SDnA₃ lactobacillus strains and CaCo-2cells was greater compared to controls, the adhesive effect between SDnB₁ and CEF cells was also greater than controls and Lactobacillus showed only minimal adherence to MDCK cells. Incubation time also affected Lactobacillus adherence to CaCo-2cells: adhesive ability was optimal at 37°C when incubated for 2 days and this was confirmed by a local increase in the concentration of Lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h. Adherence ability in lactobacillus was also tested at various concentrations (10⁸, 10⁷, 10⁶, 10⁵ and 10⁴ ). The number of Lactobacillus that adhered around the cells was significantly increased in the treatment with 10⁸ bacterial cells. Transmission electron microscopy revealed that the cellularity of the junction between CaCo-2cells and Lactobacillus was not compromised. Transmission electron microscopy also revealed that the thalli fabric structure remained intact.

Keywords

Host Specific; CaCo-2Cells; MDCK Cells; CEF Cells; High Adhesion Lactobacillus

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Adlerbertti, I., Ahrne, S., Johansson, M.L., Molin, G., Hanson, L.A. and Wold, A.E. (1996) A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Applied and Environmental Microbiology, 62, 2244-2251.
[2]	Bernet, M.-F., Brassart, D., Neeser, J.-R. and Servin, A.L. (1993) Adhesin of human bifidobacterial strains to cultured human intestinal epithelial cells and in hibition of enteropathogen cell interactions. Applied and Environmental Microbiology, 59, 4121-4128.
[3]	Barrow. P.A., Brooker, B.E. and Fuller, R. (1980) The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestinal. Journal of Applied Bacteriology, 48, 147-154. doi:10.1111/j.1365-2672.1980.tb05216.x
[4]	Bernet, M.F., Brassart, D. and Neeser, J.R. (1994) ServinAL. Lactobacillus acidopHilus LA 1 binds to Cultured intestinal cell Lines and inhibits cell attachment cell invasion by enterovirulen. Bacteria Gut, 35, 483-489.
[5]	Fontaine, I.F., Aissi, E.A. and Bouquelet, S.J.L. (1994) In vitro binding of Bifidobaeterium bifidum DSM 20082 to mucosal glycoproteins and hemagghutinating activity. Current Microbiology, 28, 325-330.
[6]	Fuller, R. and Turvey, A. (1971) Bacteria associated with the intestinal wall of the fowl (Gallus domesticus). Journal of Applied Bacteriology, 34, 617-622. doi:10.1111/j.1365-2672.1971.tb02325.x
[7]	Flint, S.H., Brooks, J.D. and Bremer, P.J. (1997) The influence of cell surface properties of thermophilic streptococci on attachment to stainless steel. Journal of Applied Microbiology, 83, 508-517. doi:10.1046/j.1365-2672.1997.00264.x
[8]	Fuller, R. (1977) The importance of lactobacilli in maintaining normal microbial balance in the crop. British Poultry Science, 18, 85-94.
[9]	Gusils, C., Palacios, J. and Gonzalez, S. (1999) Lectin-like protein fractions in lactic acid bacteria isolated from chickens. Biological and Pharmaceutical Bulletin, 22, 11-15.
[10]	Gusils, C., Cuozzo, S. and Sesma, F. (2002) Examination of adhesive determinants in three species of lactobacillus isolated from chicken. Canadian Journal of Microbiology, 48, 34-42.
[11]	Edelman, S., Westerlund-Wikstrom, B., et al. (2002) In vitro adhesion specificity of indigenous lactobacilli within the avian intestinal tract. Applied and Environmental Microbiology, 68, 5155-5159. doi:10.1128/AEM.68.10.5155-5159.2002
[12]	Mack, D.R., Michail, S., Wei, S. and Mcdougall, L.C. (1999) Probiotics inbibit enterropathogenic E. Coli adherence in vitro by inducing intestinal mucin gene expression. American Journal of Physiology, 276, G941-G950.
[13]	Rinkinen, M., Westermarck, E., Salminens, O.U. and Wehand, A.C. (2003) Absence of hose specificity for in vitro adhesion of probiotic lactic acid bacteria to intestinal mucus. Veterinary Microbiology, 97, 55-61. doi:10.1016/S0378-1135(03)00183-4
[14]	Greene Jeffreyd and Toddr Klaenhammer. (1994) Factors involved in adherence of lactobacilli to human CaCo-2 colls. Applied and Environmental Microbiology, 60, 4487- 4494.