Production of iturin A through glass column reactor (GCR) from soybean curd residue (okara) by Bacillus subtilis RB14-CS under solid state fermentation (SSF)

Abdul Wahab Khan, Umme Salma Zohora, Mohammad Shahedur Rahman, Masahiro Okanami, Takashi Ano

Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan.
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, Japan.
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.
Proximo Biotech Ltd., Dinajpur, Bangladesh.Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan.

DOI: 10.4236/abb.2012.32021                

Abstract

The present study was conducted with an aim to scale up the production of iturin A using soybean curd residue (okara). Iturin A was produced by indigenous bacterial strain Bacillus subtilis RB14-CS through glass column reactor (GCR) under solid state fermentation (SSF) was characterized. The enhanced iturin A production was observed with respect to enhanced substrate bed height when SSF was conducted in Erlenmeyer flask. To check the effect of substrate bed height on iturin A production under SSF of okara, GCR was introduced. Substrate bed height of 15 cm was suitable for iturin A production which was about 2700 mg/kg wet substrate. The observed iturin A production by the aerobic bacteria Bacillus subtilis in nearly anaerobic condition in such high substrate bed for SSF is a wonderful finding for development of SSF system in future.

Keywords

Solid State Fermentation; Okara; Iturin A; Glass Column Reactor

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Robinson, T., Singh, D. and Nigam, P. (2001) Solid-state fermentation: A promising microbial technology for secondary metabolite production. Applied Microbiology and Biotechnology, 55, 284-289. doi:10.1007/s002530000565
[2]	Mizumoto, S., Hirai, M. and Shoda, M. (2006) Production of lipopeptide antibiotic iturin A using soybean curd residue cultivated with Bacillus subtilis in solid-state fermentation. Applied Microbiology and Biotechnology, 72, 869-875. doi:10.1007/s00253-006-0389-3
[3]	Yang, S.Q., Yan, Q.J., Jiang, Z.Q., Li, L.T., Tian, H.M. and Wang, Y.Z. (2006) High-level of xylanase production by the thermophilic Paecilomyces themophila J18 on wheat straw in solid-state fermentation. Bioresource Technology, 97, 1794-1800. doi:10.1016/j.biortech.2005.09.007
[4]	Khare, S.K., Jha, K. and Gandhi A.P. (1995) Citric acid production from okara (soy-residue) by solid-state fermentation. Bioresource Technology, 54, 323-325. doi:10.1016/0960-8524(95)00155-7
[5]	Ohno, A., Ano, T. and Shoda, M. (1996) Use of soybean curd residue, okara, for the solid state substrate in the production of a lipopeptide antibiotic, iturin A, by Bacillus subtilis NB22. Process Biochemistry, 31, 801-806. doi:10.1016/S0032-9592(96)00034-9
[6]	Ohno, A., Ano, T. and Shoda, M. (1995) Effect of temperature on production of lipopeptide antibiotics, iturin A and surfactin by a dual producer, Bacillus subtilis RB14, in solid-state fermentation. Journal of Fermentation and Bioengineering, 80, 517-519. doi:10.1016/0922-338X(96)80930-5
[7]	Hiraoka, H., Ano, T. and Shoda, M. (1992) Characteristics of Bacillus subtilis RB14, copoducer of peptide antibiotics iturin A and surfactin. The Journal of General and Applied Microbiology, 38, 635-640. doi:10.2323/jgam.38.635
[8]	Phae, C. G., Shoda, M. and Kubota. H. (1990) Suppressive effect of Bacillus subtilis and its products on phytopathogenic microorganisms. Journal of Fermentation and Bioengineering, 69, 1-7. doi:10.1016/0922-338X(90)90155-P
[9]	Asaka, O. and Shoda, M. (1996) Biocontrol of Rhizoctonia solani damping off of tomato with Bacillus subtilis RB14. Applied and Environmental Microbiology, 62, 4081-4085.
[10]	Mizumoto, S., Hirai, M. and Shoda, M. (2007) Enhanced iturin A production by Bacillus subtilis and its effect on suppression of the plant pathogen Rhizoctonia solani. Applied Microbiology and Biotechnology, 75, 1267-1274. doi:10.1007/s00253-007-0973-1
[11]	Ohno, A., Ano, T. and Shoda, M. (1995) Production of a lipopeptide antibiotic, surfactin, by recombinant Bacillus subtilis in solid-state fermentation. Biotechnology and Bioengineering, 47, 209-214. doi:10.1002/bit.260470212
[12]	Phae, C.G. and Shoda, M. (1991) Investigation of optimal conditions for foam separation of iturin, an antifungal peptide produced by Bacillus subtilis. Journal of Fermentation and Bioengineering, 71, 118-121. doi:10.1016/0922-338X(91)90235-9
[13]	O’brien, J., Wilson, I., Orton, T. and Pognan, F. (2000) Investigation of the alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry, 267, 5421-5426. doi:10.1046/j.1432-1327.2000.01606.x
[14]	Ohno, A., Ano, T. and Shoda, M. (1993) Effect of temperature change and aeration on the production of the antifungal peptide antibiotic Iturin by Bacillus subtilis NB22 in liquid cultivation. Journal of Fermentation and Bioengineering, 75, 463-465. doi:10.1016/0922-338X(93)90098-S
[15]	Nakano, M.M. and Zuber, P. (1998) Anaerobic growth of a “strict aerobe” (BACILLUS SUBTILIS). Annual Review of Microbiology, 52, 165-190. doi:10.1146/annurev.micro.52.1.165