Study on Resonance Rayleigh Scattering Spectra of Mercaptopurine-Cu(II)-Nucleic Acid System

Jidong Yang, Yang Li

School of Chemistry and Chemical Engineering, Southwest China University, Chongqing, China.
School of Chemistry and Environmental Engineering, Chongqing Three Gorges University, Chongqing, China.

DOI: 10.4236/ajac.2012.33026                

Abstract

In pH 4.8 Britton-Robinson (B-R) buffer solution, Mercaptopurine (MP) could react with Cu(II) to form stable chelate compound, a new Resonance Rayleigh scattering (RRS) spectrum generated and enhanced for the binary system which the highest peak located at 453 nm as detection wavelength. But the RRS spectra could be quenched after adding her- ring milt DNA (hsDNA), salmon milt DNA (sDNA) and calf thymus DNA (ctDNA) into the binary system, respect- tively. And the weakened degree of spectra was directly proportional to the concentration of DNA. The reaction product of three nucleic acid system have identical spectral features, their range of linearity for the relation of spectral intensity with concentration respectively are 0.05 - 0.9 μg.mL^-1 for hsDNA, 0.1 - 0.9 μg.mL^-1 for sDNA and 0.3 - 0.9 μg.mL^-1 for ctDNA; their detection limit respectively are 5 ng.mL^-1for hsDNA, 6 ng.mL^-1for sDNA, 6 ng?mL^-1 for ctDNA. So a new method for determination of DNA was developed and successfully applied to determine the content of the DNA in artificial synthetized samples. At the same time the spectral features of absorption spectra and RRS spectra of the three reaction system, and the eligible reaction conditions and influencing factors were investigated in this paper.

Keywords

DNA; Mercaptopurine; Cuprum(II); Resonance Rayleigh Scattering

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	J. Gallego and G. Varani, “Targeting RNA with Small-Molecule Drugs: Therapeutic Promise and Chemical Challenges,” Accounts of Chemical Research, Vol. 34, No. 10, 2001, pp. 836-843. doi:10.1021/ar000118k
[2]	L. H. Hurley, “DNA and Its Associated Processes as Targets for Cancer Therapy,” Nature Reviews Cancer, Vol. 33, No. 2, 2002, pp. 188-200. doi:10.1038/nrc749
[3]	X. K. Teng and H. S. Xiao, “Perspectives of DNA Microarray and Next-Generation DNA Sequencing Technologies,” Science in China Series C: Life Science, Vol. 52, No. 1, 2009, pp. 7-16. doi:10.1007/s11427-009-0012-9
[4]	F. L. Jin, X. L. Dong, X. X. Xu and S. X. Ren, “cDNA Cloning and Recombinant Expression of the General Odorant Binding Protein II from Spodoptera Litura,” Science in China Series C: Life Science, Vol. 52, No. 1, 2009, pp. 80-87. doi:10.1007/s11427-009-0001-z
[5]	L. Li, Z. H. Qu, H. Wang and Z. H. Li, “Methylation Reaction for Four DNA Base Molecules by Methanedia- zonium Ions,” Science in China Series C: Life Science, Vol. 52, No. 1, 2009, pp. 26-30.
[6]	J. J. Zhang, Y. Shao, L. Wei, Y. Li, X. Sheng, et al., “Design of Artificial Nucleases and Studies of Their Interaction with DNA,” Science in China Series C: Life Science, Vol. 52, No. 4, 2009, pp. 402-414.
[7]	V. W. Rmstrong and M. Oellerich, “New Developments in the Immunosuppressive Drug Monitoring of Cyclosporine, Tacrolimus, and Azathioprine,” Clinical Biochemistry, Vol. 34, No. 1, 2001, pp. 9-16. doi:10.1016/S0009-9120(00)00175-2
[8]	Z. P. Li, K. A. Li and S. Y. Tong, “Determination for Micro Amounts of Nucleic Acids by a Resonance Light Scattering Technique with Dequalinium Chloride,” Talanta, Vol. 55, No. 4, 2001, pp. 669-675. doi:10.1016/S0039-9140(01)00423-4
[9]	S. P. Liu, X. L. Hu, H. Q. Luo and L. Fan, “Resonance Rayleigh Scattering Spectral Characteristics of Interaction of Nucleic Acids with Some Cationic Surfactants and Their Analytical Applications,” Science in China Series B: Chemistry, Vol. 45, No. 2, 2002, pp. 173-183. doi:10.1360/02yb9024
[10]	S. P. Liu, L. Fan, X. L. Hu, Z. F. Liu and Y. H. Chen, “Study on the Interaction between Proteins and Some Complexon Dyes by Resonance Rayleigh Scattering Method,” Acta Chimica Sinica, Vol. 62, No. 17, 2004, pp. 1635-1640.
[11]	G. C. Zhao, P. Zhang, X. W. Wei and Z. S. Yang, “Determination of Proteins with Fullerol by a Resonance Light Scattering Technique,” Analytical Biochemistry, Vol. 334, No. 2, 2004, pp. 297-302. doi:10.1016/j.ab.2004.07.007
[12]	S. P. Liu, H. Q. Luo, N. B. Li and Z. F. Liu, “Resonance Rayleigh Scattering Study of the Interaction of Heparin with Some Basic Diphenyl Naphthylme-thane Dyes,” Ana lytical Chemistry, Vol. 26, No. 6, 2001, pp. 3907-3914. doi:10.1021/ac001454h
[13]	H. Q. Luo, S. P. Liu and Z. F. Li, “Resonance Rayleigh Scattering Spectra for Studying the Interaction of Heparin with Some Basic Phenothiazine Dyes and Their Analytical Application,” Acta Chimica Sinica, 2001, Vol. 449, No. 1-2, pp. 267-270.
[14]	J. D. Yang, S. X. Deng, Z. F. Liu, L. Kong and S. P. Liu, “Fluorescence Quenching of Serum Albumin by Rifamycin Antibiotics and Their Analytical Application,” Luminescence, Vol. 22, No. 6, 2007, pp. 559-566. doi:10.1002/bio.1001
[15]	J. D. Yang, T. W. Cao and S. P. Liu, “Resonance Rayleigh Scattering Spectra of Rifamycins Antibio-tics-Cu(II)-Nnu- cleic Acid System,” Acta Chimica Sinica, Vol. 66, No. 19, 2008, pp. 2131-2137.
[16]	X. Q. Wang, T. Y. You, X. R. Yang and E. K. Wang, “End-Column Amperometric Detection of 6-Mercaptopurme by Capillary Zone Electrophoresis,” Chinese Journal of Analytical Chemistry, Vol. 27, No. 10, 1999, pp. 1141-1145.
[17]	J. S. Su, X. M. Chen, H. A. Luo, et al., “Determination of Deoxyribonucleic Acid with New Mma-genta by a Resonance Light-Scattering Technique,” Journal of Instrumental Analysia, Vol. 24, No. 1, 2005, pp. 60-63.
[18]	V. Vijayanathan, T. Thomas and T. J. Thomas, “DNA Nanoparticles and Development of DNA Delivery Vehicles for Gene Therapy,” Biochemistry, 2002, Vol. 41, No. 48, pp. 14085-14094. doi:10.1021/bi0203987
[19]	S. A. Woodson, “Metal Ions and RNA Folding: A Highly Charged Topic with a Dynamic Future,” Current Opinion in Chemical Biology, Vol. 9, No. 2, 2005, pp. 104-109. doi:10.1016/j.cbpa.2005.02.004