High Blood Pressure Effects on the Brain Barriers and Choroid Plexus Secretion


High blood pressure produces ventricular dilation, variations in circumventricular organs and changes in the cerebrospinal fluid compositions. On the other hand, chronic hypertension in spontaneously hypertensive rats can cause changes in the integrity of the brain barriers: blood-cerebrospinal fluid barrier and blood brain barrier. The permeability of the brain barriers can be studied by using transthyretin and S-100β. In the present work we study the integrity of the brain barrier and the choroid plexus function variations in arterial hypertension. Control rats and spontaneously hypertensive rats were used and the choroid plexus were processed by immunohistochemistry with anti-transthyretin and anti-vasopressin. Western blot was also performed in cerebrospinal fluid, serum and choroid plexus using anti-S-100β, anti-transthyretin. The accumulation of transthyretin immunoreactive was bigger in spontaneously hypertensive rats with respect to the control. Vasopressin was also higher in spontaneously hypertensive rats with respect to the control. Western blot showed that transthyretin tetramer was higher in the spontaneously hypertensive rats than in the control rats. The expression of transthyretin monomer was lower in hypertensive rats than the control in the cerebrospinal fluid, the transthyretin monomer reaction in the blood was stronger in hypertensive than in control rats. Western blot for the S-100 β showed an increase in blood and cerebrospinal fluid of hypertensive rats. The high blood pressure produces a disruption of the blood brain barrier and blood to cerebrospinal fluid barrier that allows extravasations from the cerebrospinal fluid to the blood and from the blood to the cerebrospinal fluid.

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I. González-Marrero, L. Castañeyra-Ruiz, J. M. González-Toledo, A. Castañeyra-Ruiz, H. de Paz-Carmona, L. Ruiz-Mayor, A. Castañeyra-Perdomo and E. M. Carmona-Calero, "High Blood Pressure Effects on the Brain Barriers and Choroid Plexus Secretion," Neuroscience and Medicine, Vol. 3 No. 1, 2012, pp. 60-64. doi: 10.4236/nm.2012.31009.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] I. Martínez-Pe?a y Valenzuela, E. M. Carmona-Calero, H. Pérez-González, C. Ormazabal-Ramos, P. Fernández- Rodríguez, I. González-Marrero, A. Casta?eyra-Perdomo and R. Ferres-Torres, “Alterations of the Cerebrospinal Fluid Proteins and Subcommissural Organ Secretion in the Arterial Hypertension and Ventricular Dilatation. A Study in SHR Rats,” Histology and Histopathology, Vol. 21, No. 2, 2006, pp. 179-185.
[2] I. González-Marrero, E. M. Carmona-Calero, P. Fernández-Rodríguez, H. Pérez-González, C. Ormazabal-Ramos, L. Casta?eyra-Ruiz, C. G. Pérez-García, I Martínez-Pe?a-Valenzuela, A. Casta?eyra-Ruiz, A. Casta?eyra- Perdomo and R. Ferres-Torres, “Expression of Certain Proteins in the Subfornical Organ and Cerebrospinal Fluid of Spontaneously Hypertensive Rats,” Histology and Histopathology, Vol. 22, No. 12, 2007, pp. 1371-1378.
[3] E. M. Carmona-Calero, H. Perez-Gonzalez, I. Martinez- Pe?a y Valenzuela, I. Gonzalez-Marrero, C. G. Perez-Garcia, N. Marrero-Gordillo, C. Ormazabal-Ramos, A. Casta?eyra-Perdomo and R. Ferres-Torres, “Effect of the Arterial Hypertension and Captopril Treatment on the Angiotensin II Content in the Subfornical Organ. A Study in SHR Rats,” Histology and Histopathology, Vol. 20, No. 1, 2005, pp. 135-138.
[4] H. Katsumura, M. Kabuto, K. Hosotani, Y. Handa, H. Kobayashi and T. Kubota, “The Influence of Total Body Hyperthermia on Brain Haemodynamics and Blood-Brain Barrier in Dogs,” Acta Neurochir (Wien), Vol. 135, No. 1-2, 1995, pp. 62-69. doi:10.1007/BF02307416
[5] H. Al-Sarraf and L. Philip, “Effect of Hypertension on the Integrity of Blood Brain and Blood CSF Barriers, Cerebral Blood Flow and CSF Secretion in the Rat,” Brain Research, Vol. 975, No. 1-2, 2003, pp. 179-188. doi:10.1016/S0006-8993(03)02632-5
[6] H. Al-Sarraf , F. Ghaaedi and Z. Redzic, “Time Course of Hyperosmolar Opening of the Blood-Brain and Blood-CSF Barriers in Spontaneously Hypertensive Rats,” Journal of Vascular Research, Vol. 44, No. 2, 2007, pp. 99-109. doi:10.1159/000098260
[7] H. A. Montecinos, H. Richter, T. Caprile and E. M. Rodríguez, “Synthesis of Transthyretin by the Ependymal Cells of the Subcommissural Organ,” Cell and Tissue Research, Vol. 320, No. 3, 2005, pp. 487-499. doi:10.1007/s00441-004-0997-0
[8] A. Chodobski, G. D. Silverberg and J. Szmydynger-Chodobska, “The Role of the Choroid. Plexus in the Transport and Production of Polypeptides,” In: W. Zheng and A. Chodobski, Eds., The Blood-Cerebrospinal Fluid Barrier, Chapman & Hall/CRC Taylor & Francis Group, Boca Raton, 2005, pp. 242-278.
[9] N. Marchi, V. Fazio, L. Cucullo, K. Kight, T. Masaryk, G. Barnett, M. Vogelbaum, M. Kinter, P. Rasmussen, M. R. Mayberg and D. Janigro, “Serum Transthyretin Mono- mer as a Possible Marker of Blood-to-CSF Barrier Dis- ruption,” Journal of Neuroscience, Vol. 23, No. 5, 2003, pp. 1949-1955.
[10] B. J. Blyth, A. Farhavar and C. Gee, “Validation of serum markers for bloodbrain barrier disruption in traumatic brain injury,” Journal of Neurotrauma, Vol. 26, No. 9, 2009, pp.1497-1507. doi:10.1089/neu.2008.0738
[11] F. M. Faraci and D. D. Heistad, “Regulation of Cerebral Blood Vessels by Humoral and Endothelium-Dependent mechanisms. Update on Humoral Regulation of Vascular Tone,” Hypertension, Vol. 17, No. 6, 1991, pp. 917-922.
[12] U. K. Laemmli, “Cleavage of Structural Proteins during the Assembly of the Head of Bacterio-phage T4,” Nature, Vol. 227, No. 5259, 1970, pp. 680-685. doi:10.1038/227680a0
[13] C. Capone,G, Faraco, L. Park, X. Cao, R. L. Davisson and C. Iadecola, “The Cerebrovascular Dysfunction Induced by Slow Pressor Doses of Angiotensin II Precedes the Development of Hypertension,” American Journal of Physiology, Vol. 300, No. 1, 2011, pp. H397-H407.
[14] C. E. Johanson, E. G. Stopa and P.N. McMillan, “The blood-cerebrospinal fluid barrier: structure and functional significance,” Methods in Molecular Biology, Vol. 686, 2011, pp. 101-131. doi:10.1007/978-1-60761-938-3_4

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