Epidemiology of Nosocomial Bacteremia Due to Bacteria from the “Burkholderia cepacia Complex” at Libreville University Hospital Center

Abstract

Introduction: Burkholderia cepacia is a non-fermenting emergent bacterium common in nosocomial infections and can cause life-threatening infections whose multidrug resistance makes them a serious threat in hospitals. The aim of this study was to determine the prevalence of B. cepacia infections during nosocomial infections at Libreville University teaching hospital. Methodology: In this cross-sectional study, lasting 19 months, 412 blood cultures were analyzed. The BacT/ALERT 3D (Biomerieux, France) was used to detect the positivity of blood culture flasks and the Viteck 2 compact (Biomerieux, France) for the identification of germs and the study of their susceptibility to antibiotics. Results: Our study population consisted of 412 patients. The sex-ratio M/F was 1.06 in favor of the male gender (n = 201, 51%). The age of the patients varied between 0 and 82 years. The bacteremia of B. cepacia mainly affected children under 15 years of age with a prevalence of 7% (n = 28). The pediatric ward was more represented with a frequency of 36% (n = 10). The antibiotic sensitivity profile showed high resistance of 100% for aminoglycosides (amikacin, tobramycin, and gentamycin), tetracycline, beta-lactams (Amoxicillin, Imipenem, Ticarcillin, Cefoxitin and Cefotaxime), and ciprofloxacin. However, four molecules were active on B. cepacia (Levofloxacin 100%, Trimethoprim + sulfamethoxazole 92.3%, ceftazidime 80% and cefepime 35%). Conclusion: Ultimately, infection and multi-resistance due to Burkholderia cepacia calls for a review of hospital hygiene in the pediatric ward and a review of antibiotic therapy in young children.

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Medzegue, S., Nguema, P. , Angone, S. and Rerambiah, L. (2021) Epidemiology of Nosocomial Bacteremia Due to Bacteria from the “Burkholderia cepacia Complex” at Libreville University Hospital Center. Advances in Microbiology, 11, 417-427. doi: 10.4236/aim.2021.119031.

1. Introduction

The problem of bacterial infections in hospital care is still topical and remains a major public health problem. The epidemiology of cross Infection according to the World Health Organization (WHO), 2009 showed that an average of 8.7% of hospitalized patients contracts a nosocomial infection. The report found that in the European and Western Pacific regions, the prevalence rate is 7.7% and 9.0%, respectively. This prevalence rate varies between 1.6% and 11% in Gabon [1]. The data suggest that nosocomial infections are widespread in sub-Saharan Africa, with ICU and surgical sites being the most represented. However, the etiologies of nosocomial infections and hospital unit sites are not well known in Gabon [1].

Burkholderia cepacia (B. cepacia) has become a major bacterium responsible for isolated nosocomial infections during bacteremia. This bacterium was formerly known as Pseudomonas cepacia, a Gram-negative, non-refermentable, multi-resistant aerobic bacillus.

Immunocompromised and hospitalized patients are particularly vulnerable to this bacterium with severe bacteremia leading to death [2]. B. cepacia is primarily involved in two groups of infections namely nosocomial infections (bacteremia, respiratory and urinary tract infections), most often occurring in patients subjected to invasive maneuvers (intravenous catheterization, urinary catheterization) and colonization as part of cystic fibrosis, the severity of which is very variable; Those from simple asymptomatic colonization to necrotizing pneumonia, with or without sepsis (“cepacia syndrome”) are often fatal [2]. B. cepacia is a common bacterium of nosocomial infections that can cause life-threatening infections and that, due to its multidrug resistance, is a serious threat in a hospital setting [3].

Today, emergence of multi-resistance in Gram-negative pathogens (particularly Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Acinetobacter spp. and the Enterobacteriaceae) is the main problem in medicine. In these organisms, three-component multidrug efflux systems play important roles in both intrinsic and acquired multi-resistance [4].

Moreover, in the last decade, along with the problem of nosocomial infections, multidrug-resistant bacteria in community and hospitals have soared. High frequencies of multidrug-resistant bacteria have been grouped under the acronym ESKAPE: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp. [5].

The purpose of this cross-sectional study was to determine the prevalence of B.cepacia infection at the Libreville University Hospital Center (LUHC), to identify the clinical features associated with it and to present the level of resistance of molecules commonly used in therapy.

2. Methodology

A descriptive 19-month cross-sectional study was conducted from February 2017 to October 2018, based on laboratory surveillance of cases of invasive bacterial infections due to pathogens responsible for bacteremia in the hospitalization departments of the Libreville University Hospital Center.

The study was carried out within Libreville. Libreville is located on the Gabon, a country of Central Africa. Our study population consisted of patients of both sexes and of all ages hospitalized in the different hospital departments of the University Hospital of Libreville and other health facilities of Libreville. We included in this study patients of both sexes and of any age from blood culture vials of the BacT Alert type of Biomerieux referred to the microbiology unit for suspicion of bacteremia. Not included in this work were all outpatient patients and those whose blood culture collection was done in other types of culture media other than Bact Alert vials.

All patients with bacteremia due to B. cepacia were included in the study. Patients colonized with B. cepacia in sites other than blood were excluded from the study. Data were obtained from blood culture results. Monitoring for nosocomial infection was performed on the basis of patient laboratory records during the study period. The diagnosis of nosocomial infection was made according to WHO criteria. Patient data were collected and analyzed: age, sex, department, length of hospitalization, presence of previous antibiotic treatment, microbiological data and blood culture results. The bacteria were identified using the Vitek 2 Compack technique (Biomérieux).

The antimicrobial susceptibility study of B. cepacia clinical isolates was conducted using the Viteck method with a bacterial suspension equivalent to 0.5 McFarland.

The ethics committee’s approval for the study was obtained. The analysis of the data was evaluated on the basis of a questionnaire developed. The data was captured and analyzed on Excel software and Epi Info 7 software version 7.1.3.0. The chi2 test was used for statistical analysis of variables. Continuous data were expressed as mean and standard deviation and categorical data were expressed as frequency and percentage.

3. Results

In this study, individual microorganisms were isolated from clinical samples of blood cultures test in 412 patients. The prevalence of B. cepacia as an etiological agent was 7% (n = 28) of nosocomial isolates. The data collected showed that 53% (n = 218) of blood cultures test were positive (bacteremia). Table 1 presents

Table 1. Distribution of nosocomial pathogens by departement.

*Departements: Cardiology (CARDIO); Pediatrics (PED); Resuscitation (REA); Gastroenterology (GASTRO-ENTERO); Medicine (MED); Infectious Disease (INFECTIO); Neurology (NEURO); Neonatology (NEO-NAT), Emergency (URG).

the profile of microorganisms that cause nosocomial bacteria. 28 strains of B. cepacia were isolated from blood cultures test of which 22 (79%) were male and 6 (21%) were female. The sex-ratio Male/Female was 3.66. The average age was 2.89 3.96 years. The average length of stay in hospital was 15.2 9.9 days (median 15 days). Previous antibiotic use was observed in 381 patients (38.5%).

The majority of B. cepacia infections were observed in pediatrics. The most exposed age group is children under one year of age with a frequency of 36% (n = 10). Figure 1 shows the age distribution of B. cepacia infection.

The demographic characteristics of patients with B. cepacia infection are presented in Table 2. Figure 2 summarizes the antimicrobial susceptibility of B. cepacia isolates. The most active antimicrobial agents were trimethoprim + sulfamethoxazole, levofloxacin, ceftazidime and cefidomide.

Figure 1. Distribution of B.cepacia infection by age group.

Figure 2. Antibiotic resistance profile (%) of B. cepacia isolates.

Table 2. Demographic characteristics of the population with B. cepacia.

4. Discussion

The main findings of the study were indicated: Burkholderia cepacia is found mostly in children as adults and in the department with the most inadequate hygiene (paediatrics). Burkholderia cepacia is present in patients without cystic fibrosis and without immunosuppression. The antibiotic therapy for paediatric bacteremia treatments should be revised because of the recurrence of Burkholderia cepacia in paediatrics and its multi-resistance.

B. cepacia is now considered to be a nosocomial pathogen that causes serious problems in the clinical setting due to its high inter-patient transmissibility and multidrug resistance [6].

B. cepacia has also been recognized, although rarely, as a cause of fatal disease in healthy individuals. This microorganism is associated with a wide variety of infections including pneumonia, bacteremia, skin and soft tissue infections, infection of the genito-tracturinary tract secondary to urethral instrumentation or by exposure to contaminated solutions in hospitalized patients. It is the cause of increased mobility and mortality [7] [8].

The study population consisted of 412 patients of all genders. The average age was 21.09 - 22.39 years. The most affected age group was 0 - 15 years old from pediatrics with a frequency of 55% (N = 228), these children did not have cystic fibrosis. Bacteremia to B. cepacia mainly affects children with a frequency of 7% (n = 28) and an average age of 2.89 3.96 years. The risk factors for nosocomial bacteriomial predisposition in paediatrics are: age, length of hospitalization, invasive procedures, parenteral feeding and administration of broad-spectrum antibiotics.

Our hospital data showed that the male gender was more represented (79%, n = 22) than the female gender (21%, n = 6) with a sex-ratio of H/F = 3.66. This result is superimposed on the literature which reported in 46 cases that 30.4% (n = 14) were women and 69.6% (n = 32) were men [9] [10]. However, other studies have reported that there is no difference between the two sexes [2]. Generally speaking, during bacteremia regardless of the germ, sex-ratio is in favor of the male gender. In Rabat one study reported a sex-ratio of 1.25 [11]. Other authors reported a sex-ratio of 1.28 [10]. These differences in humoral immunity exist from birth and are reinforced after puberty by estrogens [12]. The services that have the highest percentage of positive blood cultures at B.cepacia are: pediatrics (87%, n = 24), neonatal (10%, n = 3), and pediatric emergencies with 3% (n = 1).

This is not supported by Selçuk Nazik [9] who had the following results: Anesthesia Unit (19.6%, n = 9), Infectious Diseases Unit (17.3%, n = 8) and 13% (n = 6) for the Internal Medicine Intensive Care Unit and Urology Unit. On the other hand, Kyu Yeun Kim [2] found 14 paediatric cases and 35 adults. However, for Paramanantham [13], all subjects came from the neonatal department.

All these results are disparate from one study to another. These results can be explained by the difference in recruitment in the structures, the delays in the delivery of samples but also and especially by the misuse of antibiotics in self-medication [14]. In the study a total of 212 positive blood cultures (53%) were obtained. This is much higher than data from other studies such as the study conducted in Rabat by Benzriouil [9], which found a frequency of 21%, Michael Osthoff [15], in Switzerland found a frequency of 6% - 10% and Boukerouaz Amel [12] found a frequency of 31%. These variations may be related to the heterogeneity of the different hospital departments and the indications for blood cultures. This high bacteremia would certainly be due to blood culture contamination or improper sampling, hence the large number of negative coagulase staphylococcus isolated in our study.

The overall bacteriological profile was marked by a predominance of Gram-negative pathogens (33%, n = 134) compared to Gram-positive cocci 17% (n = 67). The prevalence of B. cepacia isolates (7%, n = 28) in our study corroborates with Keating and Schaffer in 2015 [10] where prevalence peaked at 7.6% in 2010 and then dropped to about 6%. However, some studies have reported much higher prevalence than ours. In Iran, strains of B. cepacia were isolated in respiratory samples with a frequency of 15.1% [16]. In Egypt, the percentage of isolates from the B. cepacia complex was 23% [17]. In the United States, the percentage isolates ofB. cepacia was 18.9% in patients without cystic fibrosis [18]. In Spain, the prevalence rate of B. cepacia strains was 14.9% [19]. Other authors reported lower prevalences than ours. In Ireland the prevalence of B. cepacia complex was 2.75%. In China, one study found that B. cepacia accounts for about 3.81% of nosocomial infections [20].

These differences can be explained by factors such as inadequate hospital hygiene, sample type, study period, study population, hospital type and geographic location [21]. The majority of B.cepacia strains showed high resistance (100%) to many antibiotics of the beta-lactam, aminoglycoside, quinolone and cycline family, respectively. These antibiotics were: (Amoxicillin, Imipenem, Ticarcilline, Cefoxitin and Cefotaxime), (Gentamicin, Tobramycin and Amikacin), Ciprofloxacin and tetracycline. This result is well above the studies reported in other countries. B. cepacias carbapenem (Imipenem) resistance isolated from nosocomial infections with cystic fibrosis was 48% - 89% [19] [22]. Di-Yong et al. [23] found 41.1% resistance. These results do not corroborate with Selçuk Nazik [7] who reported 65.2%, and Murat Dizbay [24] reported 22.23% sensitivity to imipenem.

With regard to cephalosporin resistance cefotaxitin, and cefoxitin, the result was high according to studies reported in the literature [9] [24] which have resistance (65.5%, 46.1%) for cefotaxime. Beta-alactamine resistance observed in B. cepacia species is related to the expression of a chromosomal and inducible beta-lactamase called Penna, described in 1988 by Prince et al., and Proenca et al., in 1993, in a B. cepacia isolate in the United States. The resistance of B. cepacia to aminoglycosides in our study is inconsistent with the study by Hassen et al. [25] which found sensitivity rates of 20% and 15.3% for gentamycin and that of Abdel Fattah Ahmed et al. in 2013 [3] which reported sensitivity of 15.3% and 13.3% for gentamycin and amikacin respectively.

Resistance to aminosides is due to a lack of lipopolysaccharide binding sites with reduced permeability of the external membrane and flow pumps that leads to intrinsic resistance to cationic antimicrobials, polymyxins and aminoglycosides [26]. These high rates of resistance to aminosides justify that aminosides are no longer currently recommended for probabilistic treatment of B.cepacia. With regard to resistance to ciprofloxacin, the result is similar with several studies that reported very high levels of resistance to ciprofloxacin [17] [23] [24] [25]. For resistance to tetracycline, the result corroborates with Omar et al. [17] who reported a resistance of 84.2%. However, our results disagree with Alaa Fahim Abdas, [21] and Omar et al. [17] who reported sensitivities of 60% and 5.8% respectively.

The multi-resistance of this complex to antibiotics may be explained by the fact that the species of the B.cepacia complex have the capacity to induce the production of modifying enzymes or the production of enzymes that degrade antimicrobials, alteration of the target site and changes in cell permeability [27]. Levofloxacin showed good activity (100%) to B. cepacia. This finding is consistent with a study conducted in Taiwan that reported a sensitivity of (80%) levofloxacin [28], in Babylon Alaa Fahim Abdas [19] reported a sensitivity of 73.3% Trimethoprime + sulfamethoxazole had a sensitivity of 92.3% and 73.3%, this result is in line with the literature [21] [28].

Ceftazidime is 80% active on B.cepacia strains, this finding corroborates those of Alaa Fahim Abdas [21] who have a sensitivity of 66.7%, Tseng et al. [28] reported (65%) and Avgri et al. (2009) 73.7%. Another study showed that (73.7%) of patients who received ceftazidime were cured [29]. However, this result is not consistent with that of Murat Dizbay [24] which achieved a resistance of 61.5%, and Hassen et al. [25] which had an opposite result, a resistance of 80%. Cefpimus was active on 35% of B. cepacia strains; this result is below that of Murat Dizbay (56.4%) and Selçuk Nazik (65%) [9] [24].

5. Conclusion

This work revealed a widespread spread of strains of B. cepacia primarily in children under one year of age in hospital and at the Libreville university hospital. B. cepacia is particularly found in newborns. The multiresistance of these bacteria to antibiotics commonly used for the treatment of paediatric infections calls on the need to adapt treatment regimens to local epidemiology and on a review of paediatric hospital hygiene. The problem of management of nosocomial infections due to bacteria B. cepacia lies in the difficulty of making the diagnosis given the low availability of laboratory equipment in the various health centers. Today B. cepacia should be considered as an emerging multidrug-resistant bacterium given its high frequency of isolation in care services and especially in pediatrics.

Acknowledgements

We thank Mr. MABICKA Alfred Junior for the translation of the abstract and the designer of the document. We thank the study participants for their participation in the study. The authors also acknowledge Professor Solange NZENZE.

Authors Contribution

Séverin Medzégué Nguéma conducted the study, developed the concept and study design and written the manuscript; Priest Davin Nguema conducted the study, collected and analyzed the samples; Sophie Aboughe Angone read and corrected the manuscript and interpreted data and drafted the manuscript; Léonard Kouegnigan Rerambiah supervised all the work and offered guidance. All authors have read and approved the final version of the manuscript.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

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