Introduction: The effects of performing soil observation on autonomic nerve function (ANS) and the profile of mood states (POMS) particularly for upsurge of sentiment were studied table. Methods: The subjects were divided into two groups: Group A with soil observation, and Group B without it. Soil observation was performed by smelling soil, touching the soil and other ways. Evaluation was made using heart rate change variability, POMS and visual analog scale (VAS). Results: Group A showed an increase in high frequency (HF) and the LF (low frequency)/HF ratio and a decrease in heart rate, leading to activating the functions of the parasympathetic nervous system. VAS values for “not comforted at all” indicated that the scale was lower in Group A than in Group B. In POMS, a factor of “vigor” became lower in Group B than in Group A. Discussion: It was suggested that soil observation was effective in bringing people to a state of physiological and mental relaxation and reducing stress because it raised the degree of being comforted, increased HF, decreased the heart rate and activated the parasympathetic nervous system. Conclusions: By performing soil observation, the heart rate decreased, HF increased and an upsurge sentiment became normalized.