As the development of machine vision technology, the color line-scan system is widely applied in the on-line inspection. Due to the non-uniform gray scale and color distortion of the image acquired by the system, the image correction is needed to reduce the problem of image processing and the stability system. Based on reasons mentioned above, a method that using polynomial fitting to correct the image is presented to solve the problem in this paper. The method has been used in the automatic optical inspection of PCB, and has been proved to be effective. So this method will have a potential application to the development of the color line-scan machine vision system.