DNA Fidelity: Expression of a Monocot Promoter in a Dicot Plant ()
ABSTRACT
The knowledge generated from the identification of
plant promoters has been very important for plant biotechnology development.
The use of promoters in transgenic plants allows a reasonable level of
regulating protein expression. With the application of reporter genes, such as gusA (uidA,) the production of a colored protein, β-glucuronidase, can be detected and measured both qualitatively
and quantitatively, and the activity of the promoter can be assessed. In this
work we use a promoter of an abundant banana fruit protein gene Musa
acuminata Acidic Chitinase class III a monocot species, to drive expression
of gusA in a dicot species, like
tomato. We evaluated the monocot promoter capabilities by localizing and
quantifying β-glucuronidase (GUS)
expression through fluorometric assays during tomato fruit ripening. Our
results suggest that this promoter could be used for specifically strong fruit
protein expression in dicot plants.
Share and Cite:
Suárez-Rodríguez, L. , Mason, H. , Ramírez-Cabrera, J. , Saucedo, L. , Gómez-Lim, M. , Arntzen, C. and López-Gómez, R. (2022) DNA Fidelity: Expression of a Monocot Promoter in a Dicot Plant.
American Journal of Plant Sciences,
13, 50-59. doi:
10.4236/ajps.2022.131004.
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