According to the conditions of laboratory management in colleges and universities, through the analysis on the current conditions and mode of the allocation of laboratory resources, this paper proposes a scientific management mode from the perspective of optimal allocation of resources. We should improve the efficiency of laboratory equipments, ensure the sharing of experimental resources to a greater extent, and effectively arouse the enthusiasm of experimental technicians, so as to promote the improvement of experimental teaching quality and provide the effective guarantee for the cultivation of excellent talents and the construction of “double first-class” universities in colleges and universities.