Over-Expression of MicroRNA-22 Protects Cardiomyocytes from Ischemic Injury ()
ABSTRACT
Objective: To investigate the protection effect of over-expressed MicroRNA- 22 on ischemic cardiomyocyte. Methods: Cardiomyocytes cultured in hypoxia and ischemia condition were transfected with recombinant adenovirus vector containing microRNA-22 plus a recombinant green fluorescent protein (GFP). Transduction efficiency and expression of microRNA-22 were assessed. The expression of downstream PTEN protein expression was detected. After successful transduction, cell activities including viability, nuclei proliferation and cell apoptosis were assessed. We used CCK-8 assay to assess cell activities, EdU assay to assess nuclei proliferation and Caspase-3/LDH assay to assess cell apoptosis. Results: MicroRNA-22 was transfected successfully using adeno-virus. The expression of microRNA-22 was significantly increased. The transduction efficiency reached more than 95%. Over-expression of microRNA-22 incardiomyocytes down regulated PTEN protein level. Over- expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion: Over-expression of microRNA-22 pro- tected cardiomyocytes from ischemic injury.
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Ling, L. , Ling, Z. and Gu, S. (2019) Over-Expression of MicroRNA-22 Protects Cardiomyocytes from Ischemic Injury.
World Journal of Cardiovascular Diseases,
9, 737-745. doi:
10.4236/wjcd.2019.910066.
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