Author(s)

Nedra Grira¹, Nadia Ben Abdelhafidh², Manel Ayoub¹, Rihab Sendesni¹, Bochra Adib¹, Najla Stambouli¹, Salah Othmani², Zied Aouni^1*, Chakib Mazigh¹

¹Research Unit, Biochemistry Department, Military Hospital of Tunis, Montfleury, Tunisia.
²Internal Medicine Department, Military Hospital of Tunis, Montfleury, Tunisia.

Deep vein thrombosis (DVT) is a multi-factorial disease involving both genetic and acquired risk factors. The objective of this study was to determine the frequencies of endothelial nitric oxide synthase (eNOS) gene polymorphisms G894T (rs1799983) and T-786C (rs2070744) to assess the role of these polymorphisms as a potential risk factor in the development of DVT. Methods: In this case-control study, we included 32 patients with deep vein thrombosis (DVT) and 31 healthy control subjects. Clinical characteristics were collected. Lipids plasma concentrations were determined by the colorimetric method. Genotyping for the polymorphisms was performed by restriction fragment length polymorphism (PCR-RFLP) method. Results: We had found that the eNos G894T genotype G/T was significantly increasing the risk of DTV (P = 0.042, OR = 3.9; 95% CI = 1.09 to 13.92). But no association of the eNOS T-786C variant and DVT was found. For the eNOs T-786C polymorphism, the frequency of the T/T genotype was 87.5% in patients (with an allelic frequency of T Allele equal to 91%). No significant difference was noted between the two groups (P > 0.05). Conclusion: The eNOs G894T polymorphism seems to be in association with DVT and may be considered as a risk factor, but this is not the case for the T-786C polymorphism.

Deep Vein Hrombosis, eNOS, NO, G894T Polymorphism, T-786C Polymorphism

Grira, N. , Abdelhafidh, N. , Ayoub, M. , Sendesni, R. , Adib, B. , Stambouli, N. , Othmani, S. , Aouni, Z. and Mazigh, C. (2016) Endothelial Nitric Oxyde Synthase Gene Polymorphisms in a Tunisian Deep Vein Thrombosis Group. Journal of Biosciences and Medicines, 4, 33-41. doi: 10.4236/jbm.2016.49004.