Author(s)

Carolina Álvarez^1,2*, Luis Valledor^3,4, Patricia Sáez², Manuel Sánchez-Olate², Darcy Ríos²

¹Centro Tecnológico de la Planta Forestal (CTPF), Instituto Forestal (INFOR), Sede Bío-Bío, Chile.
²Laboratorio de Cultivo de Tejidos Vegetales, Facultad de Ciencias Forestales y Centro de Biotecnología, Universidad de Concepción, Concepción, Chile.
³Laboratories of Adaption Biotechnologies, Global Change Research Centre, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
⁴Plant Physiology, Epiphysage Research Group, B.O.S. Department, Faculty of Biology, University of Oviedo, Oviedo, Spain.

In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting.

Pinus radiata, Adventitious Rooting, Protein Accumulation, Aging

Álvarez, C. , Valledor, L. , Sáez, P. , Sánchez-Olate, M. and Ríos, D. (2016) Proteomic Analysis through Adventitious Rooting of Pinus radiata Stem Cuttings with Different Rooting Capabilities. American Journal of Plant Sciences, 7, 1888-1904. doi: 10.4236/ajps.2016.714174.