Author(s)

Tamae Urai¹,   Haryanto¹, Kanae Mukai², Tatsuhiko Matsushita³, Kimi Asano¹, Yukari Nakajima¹, Mayumi Okuwa², Junko Sugama^2,4, Toshio Nakatani^2*

¹Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.
²Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kan-azawa, Japan.
³Division of Clinical Radiology Service, Kyoto University Hospital, Kyoto, Japan.
⁴Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa Uni-versity, Kanazawa, Japan.

Purpose: Not all obese people have hyperglycemia. We wondered about the healing progress in obese people without hyperglycemia. The purpose of this study is to observe the cutaneous wound healing process. Methods: Three-week-old male mice were fed high-fat diets (containing 60% fat) in the diet group, and commercial diets in the control group, ad libitum for 15 weeks. Circle-full-thickness cutaneous wounds were made on the dorsal skin of mice. From day 0 to day 15 after wounding, we analyzed wound healing process. We measured the blood concentration of leptin, and observed the distribution of leptin-positive cells in each wound. Results: Mean body weight, the areas of subcutaneous fat and visceral fat, and the weight of epididymal fat in the diet group were significantly greater than those in the control group at 15 weeks after feeding. The diet group did not feed on the diet after wounding; their body weight decreased remarkably to the level of the control group. The ratio of wound area, re-epithelialization, and collagen fibers did not differ between the diet and control groups on each day. The blood concentration of leptin in the diet group was significantly greater than that in the control group before wounding and until day 6 after wounding (day 0, 10 hour and day 1: p < 0.01, day 6: p < 0.05). Conclusion: The results show that the wound healing process is similar between obese and non-obese mice, and that the decrease in the leptin level in the obese mouse to that in the non-obese mouse may depend on the decrease of body weight of obese mouse.

Obesity, High-Fat Diet, Wound Healing, Leptin, Tension

Urai, T. , Haryanto,  . , Mukai, K. , Matsushita, T. , Asano, K. , Nakajima, Y. , Okuwa, M. , Sugama, J. and Nakatani, T. (2016) The Relationship between Cutaneous Wounds Made on Obese Mice or Those with Decreased Body Weight and Serum Leptin Level. Health, 8, 1015-1028. doi: 10.4236/health.2016.811105.