Based upon the theoretical research and educational practice on school organizational climate at home and abroad, the structural factors of organizational climate in universities were theoretically conceived. The deep interview and self-report questionnaires were adopted, and by using the analyses of exploring factors and of verifying factors, the results show that organizational climate in universities had four dimensions, i.e., administrative climate, teaching climate, learning climate and interpersonal climate, with a total of 16 sub-factors. Results show that the scale of organizational climate in university has good reliability and construct validity. It can be used as assessment tools of organization climate in the university.