Author(s)

Bertan Koray Balcioglu, Aylin Ozdemir-Bahadir, Duygu Hinc, Candan Tamerler, Berrin Erdag

Department of Mechanical Engineering, University of Kansas, Lawrence, USA; Bioengineering Research Center, University of Kansas, Lawrence, USA.
Immunogenetics Laboratory, TUBITAK Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Turkey.
Immunogenetics Laboratory, TUBITAK Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Turkey; Istanbul Technical University, Molecular Biology and Genetics, Istanbul, Turkey.

Hepatitis B virus (HBV) is one of the major causes of chronic hepatitis, cirrhosis and liver cancer. In combating HBV infections, HBV diagnosis and vaccination are therefore critical. The hepatitis B virus surface antigen (HBsAg) is a key target molecule in developing vaccines and diagnostic systems. To date, although HBsAg has been expressed in bacteria, yeasts and mammalian cells, there are still limitations in the existing ones, which leave the necessity for searching new HBsAg production methods. In this study, a simple phage display-based method was developed to produce the purified full-length HBsAg molecules for further immunization studies. For this purpose, the HBsAg coding gene was cloned into a pCANTAB5E phagemid vector and expressed on the surface of M13 filamentous phages. The HBsAg-expressing phage nanosystem was then used as immunization agent in BALB/cJ mice. The ELISA results for sera obtained from mice immunized with HBsAg-displaying phage particles revealed an immune response against HBsAg. These results demonstrate the potential use of a full-length antigen to be displayed on phages as cost effective adjuvant-free immunization agents as an alternative to the highly purified and more expensive antigens conjugated with carrier molecules.

Phage Display; Hepatitis B Virus Surface Antigen; Protein Expression; Phage Immunization; Nano Vector System

Balcioglu, B. , Ozdemir-Bahadir, A. , Hinc, D. , Tamerler, C. and Erdag, B. (2014) Cost effective filamentous phage based immunization nanoparticles displaying a full-length hepatitis B virus surface antigen. Advances in Bioscience and Biotechnology, 5, 46-53. doi: 10.4236/abb.2014.51008.