Author(s)

Christian Nanga Chick^1*, Mohammed Mahdaly¹, Phillippe Belle Ebanda Kedi^2,3, Kiuchi Sonoko⁴, Francois Eya’ane Meva^2,5

¹Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo, Japan.
²Laboratory of Innovative Nanostructured Material (NANO: C), Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala, Cameroon.
³Nanosciences African Network, iThemba LABS-National Research Foundation, Cape Town, South Africa.
⁴Department of Studies in Human Life Sciences, Otsuma Women’s University, Tokyo, Japan.
⁵Institute for Inorganic Chemistry and Structural Chemistry, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.

In this work, AgCl nanoparticles were synthesized from Microsorum scolopendria (MS) aqueous extract and AgNO₃ solution. Preliminary confirmation was a color change from a light brown to a dark-colored solution and a UV-Vis spectra surface plasmon resonance peak at 427 nm. Measured vibrational frequencies at 1713 cm⁻¹ and 1030 cm⁻¹ for C-O stretching of carboxylic acid or aliphatic ketone, and 1547 cm⁻¹ for possibly N-O stretching of nitro compounds by Infrared (FTIR) analysis explain the possible biomaterial electronegative species or functional groups responsible for the reduction of Ag ( 1) to Ag (0) for the formation of MS-AgCl nanoparticles. XRD analysis studies revealed that these particles contained face-centered cubic crystallites of metallic AgCl of 100 % with an average calculated crystallite size range of 30.34 nm (SD = 5.10 nm) by Scherrer’s equation and a calculated crystallite size of 66.04 nm with a lattice strain of 0.00175 nm by Williamson Hall equation. The measured albumin denaturing activity of MS-AgCl nanoparticles gave an IC₅₀ value of 26.70 µg/mL and 1.35 µg/mL for the positive control diclofenac. Additionally, the measured ability of phosphomolybdate complex formation, the antioxidant IC₅₀ value of MS-AgCl nanoparticles was 35.29 µg/mL, and positive control ascorbic acid was 13.91 µg/mL. In all, using MS fern frond aqueous extracts, this preliminary work confirms MS-AgCl nanoparticles as potential therapeutic agents for oxidative stress, inflammatory problems, and related diseases.

Microsorum scolopendria, SNPs, IR, UV-Vis, Antioxidant

Chick, C. , Mahdaly, M. , Kedi, P. , Sonoko, K. and Meva, F. (2024) Calculating the Crystallite Size of Microsorum scolopendria AgCl Nanoparticles and Their Biological Activities. Journal of Biomaterials and Nanobiotechnology, 15, 25-37. doi: 10.4236/jbnb.2024.152002.