Author(s)

Néstor Méndez Palacios^1*, Netzi Naidí Mendez Palacios¹, Felicitas Vázquez Flores¹, José Alfredo Galicia Domínguez¹, Edgar Guadalupe Beltrán Rosas², Maximino Méndez Mendoza¹

¹Facultad de Medicina Veterinaria and Zootecnia-Benemérita, Universidad Autónoma de Puebla, Puebla, Mexico.
²Independent Advisor in the Porcine Area, Puebla, Mexico.

The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FMVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 10⁶ sperm, the PRRS virus strain was ATCC-VR-2332 (0, 10², 10⁴ and 10⁶ copies of RNA/mL in triplicate), it was observed daily at the CASA; Hamilton Thorne^®. Cells with MT (P < 0.05) on days 1, 3, 5, 7 and 10 of evaluation with 201 ± 7.3, 167 ± 10.1, 165 ± 14.6, 134 ± 8.2 and 120 ± 8.8, respectively. The % MP between control and virus concentrations (P ≥ 0.05). The LCV on day 1 and 7 PI at 10X² and 10X⁶ (P < 0.05) vs control. In the Correlation Matrix, where it is observed that there is a correlation between VSL and VAP, VSL and VCL, VCL and ALH, VAP with ALH. There is a correlation of VSL and ALH, STR and ALH. In this study there were (P ≤ 0.01) in the VCL, in the concentrations (10²) 162.81 ± 10.65 and (10⁶) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 10², 10⁴ and 10⁶ of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 10⁴ with 83.35 ± 2.41 and 10⁶ with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the ALH there is (P < 0.05) a 10⁴ with 8.70 ± .26 and 10⁶ with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ejaculates with the PRRS virus affects sperm motility on days 1, 3, 5, 7, and 10 post-infections.

PRRSV, Boar Sperms, Velocity Pattern and Mobility Pattern

Palacios, N. , Palacios, N. , Flores, F. , Domínguez, J. , Rosas, E. and Mendoza, M. (2024) Pig Reproductive and Respiratory Syndrome Virus (PRRSV) Does Not Attach to Boar Sperm; It Affects Only the Velocity Pattern and the Mobility Pattern. Journal of Biosciences and Medicines, 12, 216-228. doi: 10.4236/jbm.2024.124018.