Author(s)

Patrick Hervé Diboue Betote^1,2,3*, Moustapha Gambo Abdoulaye⁴, Francis Ngolsou^3,5, Esther Del Florence Ndedi Moni², Adeline Sabine Fanta Yadang¹, Fidel Castro Lah Weyepe¹, Gabriel A. Agbor¹, Nga Nnanga^3,5,6, Maximilienne Ascension Nyegue²

¹Laboratory of Pharmacology and Drugs Discovery, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon.
²Laboratory of Microbiology, Department of Microbiology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
³Multidisciplinary Laboratory, Department of Galenical Pharmacy and Pharmaceutical Law, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
⁴Department of Physicochemical and Pharmaceutical Sciences, Faculty of Health Sciences, Abdou Moumouni University, Niamey, Niger.
⁵Department of Pharmaceutical and Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon.
⁶Laboratory of Pharmaceutical Technology, Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon.

Pneumonia, a respiratory infection induces acute or chronic inflammation, characterized by increased activity of lymphocytes and neutrophils, thus generating oxygen-free radicals that decrease the endogenous antioxidants defence system. The aim of this experimental study focused on the capacity of nontoxic aqueous, hydroethanolic and ethanolic extracts of Greenwayodendron suaveolens (Engl. & Diels) Verdc. subsp. suaveolens to regulate free reactive species and protein inflammation generated by infectious disease. The phytochemical screenings of G. suaveolens extracts were carried out according to precipitation and colorimetric methods. The total antioxidant and flavonoid contents were determined by the Folin-Ciocalteu and Aluminium Chloride ethanolic methods. The efficiency of G. suaveolens extracts on free radicals was evaluated using DPPH^•, ABTS^+•, and FRAP methods. The anti-inflammatory properties of extracts were evaluated according to in vitro protein (BSA) denaturation, Proteinase Inhibitory Action, and Red Blood Cell Membrane stabilization assays. The G. suaveolens aqueous, hydroethanolic and ethanolic extracts were used for the acute toxicity assessment according to the OECD protocol. The obtained results showed the presence of flavonoids, phenols, polyphenols, tannins, anthocyanins, alkaloids, terpenoids, and sterols as secondary metabolites families in G. suaveolens extracts. The highest contents of total antioxidants and flavonoids were highlighted in the hydroethanolic extract. However, it’s the G. suaveolens aqueous extract that showed the best free radical DPPH^• and ABTS^+• scavenging activities (SC₅₀) of 11.06 μg/mL and 15.16 μg/mL respectively. The highest ferric-reducing activity was found in G. suaveolens ethanolic extract with 866.23 μg EGA/mg of dry weight. The hydroethanolic extract has shown a high anti-inflammatory activity through BSA denaturation and erythrocyte membrane haemolysis with inhibitory concentrations 50 (IC₅₀) of 48.63 and 59.22 μg/mL respectively. In contrast, proteinase inhibitory activity revealed a better potential of IC₅₀ (34.19 μg/mL) for the ethanolic extract. In oral acute toxicity, all treated groups revealed neither mortality nor any significant alteration in behaviour and locomotion. The lethal dose 50 (LD₅₀) of G. suaveolens extracts was >5000 mg/kg. These results suggest that G. suaveolens stem-barks extracts may serve as therapeutic sources to prevent inflammation induced by oxidative stress, an important feature of infectious diseases.

Greenwayodendron suaveolens, Secondary Metabolites, Oxidative Stress, Antioxidant Activity, Anti-Inflammatory Properties and Oral Acute Toxicity

Betote, P. , Abdoulaye, M. , Ngolsou, F. , Moni, E. , Yadang, A. , Weyepe, F. , Agbor, G. , Nnanga, N. and Nyegue, M. (2024) Modulation of the in vitro Oxidative Stress and Erythrocyte Cell Membrane Integrity Using Aqueous, Hydroethanolic and Ethanolic Stem-Barks Extracts of Greenwayodendron suaveolens (Engl. & Diels) Verdc. Pharmacology & Pharmacy, 15, 39-61. doi: 10.4236/pp.2024.153004.