Author(s)

Waleed Alhussaini^1*, Ezzeldeen Ghanem¹, Magd Alsahly¹, Amani Kurdi¹, Eman Alharbi¹, Imadul Islam¹, Majed Aljeraisy²

¹Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia.
²Research Office, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia.

A sensitive, accurate and robust Liquid Chromatography Tandem Mass Spectrometry method has been developed and validated to measure voriconazole trough levels in human plasma. The plasma samples were mixed with fluconazole as an Internal Standard and directed to protein precipitation and drug extraction. An aliquot of 1 μl was injected into the chromatographic system and separated by the Acquity BEH C18 column at a flow rate of 0.30 ml/min in a gradient mobile phase consisting of acetonitrile, Ultrapure water (UPW), methanol and formic acid. Voriconazole was detected by a Triple Quadrupole Detector (TQD) operating on Multiple Reaction Monitoring (MRM) and a positive ion mode Electrospray ionization (ESI) Q1 mass: 350.1 m/z, Q3 mass: 281.1 m/z. Method linearity of the calibration curve (0.10 - 8.00 μg/ml) indicated a correlation coefficient r ≥ 0.99. The intra and inter-assay accuracy was within 85% - 115% and the intra and inter-assay precision was ≤5.76%. Voriconazole recovery percentage was between 97.69 - 119.62%. The method was successively applied in routine voriconazole TDM.

Voriconazole, Human Plasma, Liquid Chromatography, Tandem Mass Spectrometry, Therapeutic Drug Monitoring

Alhussaini, W. , Ghanem, E. , Alsahly, M. , Kurdi, A. , Alharbi, E. , Islam, I. and Aljeraisy, M. (2023) Determination of Voriconazole in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry: Application in Therapeutic Drug Monitoring. American Journal of Analytical Chemistry, 14, 378-389. doi: 10.4236/ajac.2023.149021.