Vegetation of different heights commonly grows in natural rivers, canals and wetlands and affects the biodiversity and morphological process. The role of vegetation has drawn great attention in river ecosystems and environmental management. Due to the complexity of the vegetated flow, most previous research focuses on the effect of uniformed one-layered vegetation on the flow structure and morphological process. However, less attention was paid to the impact of the mixing vegetation of different heights, which is more realistic and often occurs in natural riverine environments. This paper aims to investigate the effect of mixing three-layered vegetation on flow characteristics, particularly the velocity distrbution, via a novel experiment. Experiments were performed in a titling water flume fully covered with vegetation of three heights (10, 15 and 20 cm) arranged in a staggered pattern, which is partially submerged. Velocities at different positions along a half cross-section were measured using a mini propeller velocimeter. Observed results showed that the velocity has a distinct profile directly behind vegetation and behind the vegetation gap. The overall profile has two distinct reflections about ? below or near the top of short vegetation (h): the velocity remains almost constant in the bottom layer (<0.75 h) and then fast increases until the top of short vegetation; after a gradual increase, the velocity rapidly increases to the water surface. Moreover, in the upper layer (z > h) the velocities directly behind the middle after short vegetation increase much faster than those directly behind the short after tall vegetation. The finding in this study would help river riparian and ecosystem management.