Influence of Follicular Fluid on in Vitro Maturation and Fertilization of Bovine Oocytes ()
ABSTRACT
The aim of this study was to investigate the effect of time on in
vitro maturation
of bovine oocytes and of the addition of follicular fluid on meiotic
progression. The cumulus-oocyte complexes (COCs) collected from 3 to 6 mm
follicles were obtained from ovaries of slaughtered female animals. The medium
of maturation was supplemented or not with 20 μL follicular fluid (FF); 661
oocytes were matured in vitro (extrusion of the first polar corpuscle)
for 22 hours with added follicular fluid (AFF) (72.01%) or without
follicular fluid (WFF) (67.53%) and 679 oocytes were matured in
vitro for 26 hours (extrusion of the first polar corpuscle) with
AFF (92.1%) and WFF (77.15%). The results of extrusion of the second polar
corpuscle as an event related to the fertilization percentages showed that the
increase in the fertilization rate is maintained at 26 hours with AFF (79.45%),
but the percentage decreases WFF (65.08%). After 22 hours, the fertilization rate
was 62.38% AFF and 53.40% WFF. The developmental competence of bovine oocytes
is affected by the duration of maturation in vitro and
the inclusion in the FF culture medium. The use of follicular fluid in the in
vitro maturation
medium may be a biological strategy to increase the cumulus expansion, the
nuclear maturation and the in vitro fertilization.
Share and Cite:
Covelo, I. , Puente, M. and Tartaglione, C. (2022) Influence of Follicular Fluid on
in Vitro Maturation and Fertilization of Bovine Oocytes.
Open Journal of Animal Sciences,
12, 118-128. doi:
10.4236/ojas.2022.121009.