Author(s)

Xueyan Lin, Tian Zhang, Lin Ju, Yue Jiang, Qiuling Hou, Zhiyong Hu, Yun Wang, Zhonghua Wang^*

College of Animal Science, Shandong Agricultural University, Tai’an, China.

For the experiment, 8 newborn male Holstein calves were selected that had the same feeding environment, and were of similar ages. They were randomly divided into 2 groups, with 4 in each group. The treatments consisted of feeding active probiotics (Group P) and a normal fed control group (Group C). The growth performance and blood indices were measured; rumen fluid samples were collected after weaning, and 16SrDNA sequencing and LC-MS metabolome detection were performed. Compared with the control group, relative abundances of Deltaproteobacteria, Desulfovibrionales, Bacteroidales_ BS11_gut_group, Desulfovibrionaceae, Bacteroidales_S24-7_group, Acetobacteraceae, Ruminococcaceae_NK4A214_group, Asaia, [Ruminococcus] gauvreauii_group, Desulfovibrio, Kingella, Selenomonas, Lachnoclostridium in group P were significantly different (P < 0.05). In group P, the metabolite of 2-methylbenzoic acid and myo-inositol were significantly increased (P < 0.05). These results showed that compared with normally fed calves, the growth performance and blood indices of probiotic-fed calves were changed, but the differences were not significant. Probiotic-fed calves showed significant differences in rumen fluid and a small number of metabolites, which were mainly involved in the pathway of carbohydrate metabolism. It proves that the supplemental active probiotics had an effect on the rumen microflora.

Calves, Probiotics, Rumen Fluid, Serum, Metabolites

Lin, X. , Zhang, T. , Ju, L. , Jiang, Y. , Hou, Q. , Hu, Z. , Wang, Y. and Wang, Z. (2021) Effects of Supplemental Feeding of Probiotics during Lactation on Rumen Microflora of Calves after Weaning. Advances in Bioscience and Biotechnology, 12, 213-228. doi: 10.4236/abb.2021.127014.