Author(s)

Aiju Zhang, Zhiming Zhou

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Research Center of East China Sea Fishery Research Institute, Zhejiang Institute of Freshwater Fisheries, Huzhou, China.

Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca²⁺ out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca²⁺-ATPase gene was isolated from the gill of Hyriopsis cumingii (HcPMCA) by using SMART RACE technique. The entire cDNA was 5230 bp, including a 417-bp 5'-UTR, a 3588-bp ORF and a 1225-bp 3'-UTR, encoding a 1195-amino acid protein, and no putative signal peptide was predicted. Compared with PMCA homologs from seawater mollusks, HcPMCA had high similarity with them in both sequence and structure. Tissue-specific expression analysis revealed that HcPMCA mRNA was detected in all the sampled tissues, but was prominently expressed in the gill and mantle. When exposed to a serie of increasing Ca²⁺ that lasted for 7 days, the mRNA expression of HcPMCA in the mantle was slightly downregulated, but peaked at 60 mg/L. Moreover, the temporal expression of HcPMCA transcripts in the mantle after 60 mg/L Ca²⁺ exposure was shown to be bell-shaped, which was slightly downregulated at 24 h, but upregulated from 24 h to 48 h post-treatment, peaking at 48 h. The result of present study provides useful information for further studies on function and regulation mechanism of HcPMCA gene.

Hyriopsis cumingii (Lea), HcPMCA, Cloning, Gene Expression, Calcium Stimulation

Zhang, A. and Zhou, Z. (2021) Characteristics of a Critical Calcium Transportation Regulator: Plasma Membrane Ca²⁺-ATPase Involved in Calcium Homeostasis from Hyriopsis cumingii (Lea). American Journal of Molecular Biology, 11, 38-50. doi: 10.4236/ajmb.2021.112004.