Author(s)

Daiki Ito¹, Takanari Ikeda¹, Keijiro Taga¹, Yasushi Yamamoto^1*, Zameer Shervani², Masato Yamamoto³

¹Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan.
²Nanomaterials Production Division, Food and Energy Security Research and Product Center, Sendai, Japan.
³Department of Chemistry, School of Arts and Sciences, Showa University, Kamiyoshida Fujiyoshida, Yamanashi, Japan.

We have investigated the morphology of dimyristoyl phosphatidyl choline (DMPC)—cholesterol mixed monolayer formed on the water surface by dropping method using surface tension measurement (STm), Brewster angle microscopy (BAM), and infrared external reflection spectroscopy (IERS). STm results showed negative deviation of the limiting molecular area (A₀) of cholesterol occurred when cholesterol was added to the DMPC monolayer. BAM images showed the expandable DMPC monolayer changed to the condensed rigid monolayer at more than cholesterol mole fraction (x_Chol) 0.4. IERS recordings showed that the addition of cholesterol at x_Chol = 0.4 occurred structural change from gauche- to trans- conformation of two DMPC molecule alkyl chains. From these results, it is found that cholesterol molecule has specific properties that cause structural transition of DMPC molecule alkyl chains.

Dimyristoyl Phosphatidyl Choline (DMPC), Cholesterol, Surface Tension Measurement (STm), Brewster Angle Microscopy (BAM), Infrared External Reflection Microscopy (IERS), Structural Transition

Ito, D. , Ikeda, T. , Taga, K. , Yamamoto, Y. , Shervani, Z. and Yamamoto, M. (2019) Structural Change in Dimyristoyl Phosphatidyl Choline (DMPC) Molecule Alkyl Chains on the Addition of Cholesterol to DMPC Monolayer: Specific Addition Effect of Cholesterol. Journal of Biophysical Chemistry, 10, 15-29. doi: 10.4236/jbpc.2019.102002.