Author(s)

Nicoletta Libera Lilli¹, Delphine Révy², Christelle Volteau¹, Sandra Robelet², Béatrice Lejeune³

¹Clinical & Preclinical Development and Regulatory Affairs, Labo’ Life France, Nantes, France.
²Syncrosome, Campus Luminy-Luminy Entreprises, Marseille, France.
³Clinical & Preclinical Development and Regulatory Affairs, Labo’ Life Belgium, Parc Scientifique CREALYS, Gembloux, Belgium.

The aim of the present study was to investigate the effect of one capsule of the micro-immunotherapy medicine (MIM) 2LMISEN^® compared to vehicle, in a neuronal aging model. Senescence and apoptosis of hippocampal neurons were evaluated by measuring p16^INK4a and caspase 3 levels, respectively. The data presented is a single observation. Mice hippocampal neuron cultures were treated with MIM (11 mM) or vehicle (11 mM) from 22 days in vitro (DIV) until 27 DIV. After incubation, hippocampal neuron cultures were fixed at 15 (control condition), 22, 25 and 27 DIV and then incubated with primary antibodies p16^INK4a, MAP2 and Caspase 3. Quantification of p16^INK4a and Caspase 3-positive neurons was done using Developer software. We found that vehicle had no effect on p16^INK4a expression, whereas MIM was able to decrease p16^INK4a levels at 22, 25 and 27 DIV in a statistically significant manner. The MIM had no significative effect on Caspase 3 expression. Our preliminary results showed that the MIM capsule significantly reduced neuronal senescence and not apoptosis.

Neurons, Senescence, Micro-Immunotherapy, Low Dose

Lilli, N. , Révy, D. , Volteau, C. , Robelet, S. and Lejeune, B. (2019) Effect of 2LMISEN^® on Long-Term Hippocampal Neurons Culture as a Screening Senescent Cells Model: p16^INK4A and Caspase 3 Quantification. Advances in Aging Research, 8, 155-164. doi: 10.4236/aar.2019.86012.