Development of Disposable Single-Use Biosensor for Fructosyl Valine and Glycated Hemoglobin A1c

HTML  XML Download Download as PDF (Size: 865KB)  PP. 45-53  
DOI: 10.4236/jst.2019.94005    688 Downloads   2,191 Views  Citations

ABSTRACT

A novel amperometric biosensor prototype was fabricated using screen printing technique. The disposable single-use strips were made from conductive carbon ink and modified with fructosyl amino acid oxidase. The electrodes and conducting paths were made solely with carbon ink and characterized by conductivity and cyclic voltammetry. The biosensor showed high current output, large linearity, and effectiveness for fructosyl valine as well as human blood samples. Amperometric studies were carried out using both fructosyl valine and human blood samples. With 5 uL sample volume, the biosensor showed strong amperometric response with good linearity for a wide range (0 to 8 mM). Diabetic and healthy blood samples showed sufficient difference in their amperometric responses that correlate well with their different hemoglobin A1c levels. These results demonstrate the feasibility of using this type of inexpensive single-use biosensor strips as the basis for determining hemoglobin A1c levels for diabetic patients.

Share and Cite:

Liu, S. , Leng, J. and Aquino, T. (2019) Development of Disposable Single-Use Biosensor for Fructosyl Valine and Glycated Hemoglobin A1c. Journal of Sensor Technology, 9, 45-53. doi: 10.4236/jst.2019.94005.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.