Effects of Mitochondrial DNA Deletion and OSW-1 on PI3K-AKT Signaling Pathway PCR Array in SK-Hep1 Cells

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DOI: 10.4236/cm.2019.103006    556 Downloads   1,155 Views  Citations

ABSTRACT

To investigate the effect of mitochondrial DNA deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1 hepatocellular carcinoma cells, we prepared SK-Hep1 cells with mtDNA deletion, that is, p0SK Hep. Then the OSW-1 of 100 ng/L was used to intervene SK-Hep1 and p0SK-Hep1. RT-qPCR was used to detect the difference of gene expression on PI3K-AKT signaling pathway PCR Array in four groups of cells. The gene expression of TLR4, FOS and TSC2 markers in SK-Hep1 cells treated with OSW-1 were significantly increased. The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markers were significantly increased in p0SK-Hep1 cells, and the gene expressions of IRAK1 and GJA1 markers were significantly increased in p0SK-Hep1 cells treated with OSW-1. OSW-1 mainly affects the genes related to TLR4 pathway on PI3K-AKT signaling pathway PCR Array in SK-Hep1 HCC cells. P0SK-Hep1 mainly affects the upstream PDK1 gene and downstream TSC2 gene of Akt on PI3K-AKT signaling pathway PCR Array, and also affects the gene expression of gap junction at the same time.

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Du, X. , Qian, C. and Jin, X. (2019) Effects of Mitochondrial DNA Deletion and OSW-1 on PI3K-AKT Signaling Pathway PCR Array in SK-Hep1 Cells. Chinese Medicine, 10, 107-119. doi: 10.4236/cm.2019.103006.
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