Author(s)

Rabah Saïdi¹, Safaâ Rahmouni², Zineb Nejjar El Ansari², Abdelfettah Maouni¹, Alain Badoc³, Ahmed Lamarti^2*

¹Department of Matter and Life Sciences, High Normal School, Martil, Morocco.
²Laboratory of Plant Biotechnology, Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco.
³Axe MIB (Molécules d’Intéret Biologique), Unité de Recherche (Enologie EA 4577, USC 1366 INRA), UFR des Sciences Pharma-ceutiques, Université de Bordeaux, ISVV (Institut des Sciences de la Vigne et du Vin), Bordeaux, France.

In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l); high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA₃, while rooting is mainly favored by IBA, especially at 2 mg/l.

Carob, Ceratonia siliqua L., Cytokinins, Shoot Tip Culture

Saïdi, R. , Rahmouni, S. , El Ansari, Z. , Maouni, A. , Badoc, A. and Lamarti, A. (2019) Effect of Cytokinins on the Micropropagation of Carob (Ceratonia siliqua L.) through Shoot Tip Culture. American Journal of Plant Sciences, 10, 1469-1481. doi: 10.4236/ajps.2019.109104.