Author(s)

Yangjunqi Wang^1*, Jintao Li^1*, Fan Li¹, Shuying Li², Rugang Zhong¹

¹Beijing Key Laboratory of Environmental & Viral Oncology, College of Life Science & Bioengineering, Beijing University of Technology, Beijing, China.
²Department of Pathogenic Biology, College of Basic Medicine, Hebei United University, Tangshan, China.

Human papillomavirus is an important cancer factor in many cancers. E6E7 is the important viral oncogene and plays an important role. It is known that its function is to regulate p53 and pRb. In this paper, in situdetection was performed to determine the interaction between them; the research provides visual evidence of their interaction. Research methods: In situ PLA reaction was used to detect the relationship between two different protein. Results: The quantity of the HPV18E6 expression is much higher than the expression of HPV18E7 protein in HeLa and EC109 cells; the interaction between p53-E6 and pRb-E7 was clearly observed and this effect could be visualized by this method. Conclusion: Interaction of the HPV18E6 protein combined with p53 protein and HPV18E7 protein combined with pRb protein could be visualized in cell.

HPV E6, HPV E7, p53, pRb, PLA

Wang, Y. , Li, J. , Li, F. , Li, S. and Zhong, R. (2019) In Situ Detection of the p53-E6 and pRb-E7 Complex Formation in EC109 Cell by Proximity Ligation Assay. Journal of Cancer Therapy, 10, 336-344. doi: 10.4236/jct.2019.104027.