Study on Optimizing Nutrition and Fermentation Conditions for Compound Bacillus spp. - American Journal of Molecular Biology

AJMB > Vol.9 No.2, April 2019

Study on Optimizing Nutrition and Fermentation Conditions for Compound Bacillus spp. ()

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DOI: 10.4236/ajmb.2019.92007 1,244 Downloads 2,895 Views Citations

Author(s)

Yanyan Li^1,2, Yuxiang Xu³, Wenhao Li³, Yong Yang³, Lin Wang⁴, Jun Yu¹, Changjun Wang¹, Xihong Li^1*

Affiliation(s)

¹Tobacco Research Institute of Hubei Province, Wuhan, China.
²The Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
³Hubei University, Wuhan, China.
⁴Hubei Tobacco Industry Co., Ltd., Wuhan, China.

ABSTRACT

In order to improve the spore yield of compound Bacillus spp. (B. amyloliquefaciens, B. laterosporus and B. megaterium), the effects of nutrient conditions including carbon source, nitrogen source, mineral salt and fermentation conditions including the inoculum age, inoculation amount, loading volume of liquid and initial pH on the spore yield were studied. The results indicated that the optimized medium was glucoses 20 g/L, soybean meal 30.0 g/L, K₂HPO₄ 1.0 g/L; fermentation temperature is 37℃, the inoculum age 12 h, initial pH 7.0, 2% inoculation amount, loading volume of liquid 20 mL/250 mL. Under the optimized conditions of culture medium and fermentation for compound Bacillus spp., spore yield was 10.24 times more than the initial medium, and the spore formation rate reached more than 90%.

KEYWORDS

Compound Bacillus spp., Spore Yield, Medium Optimization, Fermentation Optimization

Share and Cite:

Li, Y. , Xu, Y. , Li, W. , Yang, Y. , Wang, L. , Yu, J. , Wang, C. and Li, X. (2019) Study on Optimizing Nutrition and Fermentation Conditions for Compound Bacillus spp.. American Journal of Molecular Biology, 9, 75-84. doi: 10.4236/ajmb.2019.92007.

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