Author(s)

Leonardo Martins Leal^1*, Alessandra Regina Scavone Ferreira², Tais Harumi de Castro Sasahara³, Aliny Antunes Barbosa Lobo Ladd³, Paola Castro Moraes², Márcia Rita Fernandes Machado²

¹Ingá University Center (UNINGá), Maringá, Brazil.
²University of the State of Sao Paulo (UNESP), Jaboticabal, Brazil.
³University of Sao Paulo (USP), Sao Paulo, Brazil.

This study aimed to quantitative evaluate the implantation of paca’s peritoneum, a new biomaterial option, preserved in 300% supersaturated sugar solution or 98% glycerin on the abdominal wall of Wistar rats. 60 males, weighing between 150 and 200 g, are organized into the following experimental groups: control group (GI); group peritoneum preserved in 300% supersaturated sugar solution (GII); and group peritoneum preserved in 98% glycerin (GIII). Each group had 20 animals. Five rats from each group underwent euthanasia at four different moments: 7^th, 15^th, 30^th, and 60^th post-operatory day for histological and stereological evaluations of the graft/native tissue interface. On histological examination, one found out an extensive presence of inflammatory infiltrate at the early periods (7^th and 15^th day) and an extensive presence of connective tissue at the late moments (30^th and 60^th day). Through stereological analysis, one found out that the number of mononuclear cells decreased throughout the evaluation moments. One concluded that the paca’s peritoneum as a biological membrane preserved in 98% glycerin, when implanted on the abdominal wall of rats, microscopically allowed one to obtain, through stereological analyses, a lower inflammatory response than the paca’s peritoneum preserved in 300% supersaturated sugar solution.

Healing, Pathology, Graft, Wild, Stereology

Leal, L. , Scavone Ferreira, A. , de Castro Sasahara, T. , Ladd, A. , Moraes, P. and Machado, M. (2018) Quantitative Evaluation of Inflammatory Response in Rats Submitted to the Abdominal Wall Surgery Using Peritoneum of Paca Preserved in 300% Supersaturated Solution of Sucrose or 98% Glycerin. Open Journal of Animal Sciences, 8, 439-449. doi: 10.4236/ojas.2018.84033.