Author(s)

Raquel Fornazari¹, Óscar Mateus², Teresa Correia^2,3, Hélder Quintas^2,3, Raimundo Maurício², Anderclei Conradi¹, Lucas Francisco¹, Armindo Álvaro⁴, Ramiro Valentim^2,3

¹Federal Technology University of Paraná, Campus of Dois Vizinhos, Dois Vizinhos, Brasil.
²Department of Animal Science, Agrarian Superior School of Braganza, Braganza, Portugal.
³Mountain Investigation Center, Polytechnic Institute of Braganza, Braganza, Portugal.
⁴Polytechnic Superior Institute of Kwanza Sul, Sumbe, Angola.

This paper aims to study the efficiency of two short-term progestagen (FGA vs. MAP) + eCG treatments in estrus synchronization and artificial insemination (AI) with fresh or chilled semen in Assaf ewes fertility rate. All ewes received a subcutaneous implant of exogenous melatonin 45 days before been treated with short-term progestagens + eCG. By June 1^st, ewes were divided in two groups: half was treated with an intravaginal sponge impregnated with 20 mg of FGA and the other half with an intravaginal sponge impregnated with 60 mg of MAP. Progestagen treatments lasted for 6 days. At sponge withdraw, all ewes were injected with 750 IU of eCG. Ovarian activity was assessed by plasmatic progesterone levels before and after progestagens + eCG treatment. Semen was collected by electro ejaculation and extended with Andromed^® or OviXcell^®. AI was performed 55 hours after eCG administration with fresh or chilled semen. During AI several factors were assessed: vagina mucosa color and lubrication, external cervical Os type, cervical mucous viscosity, semen deposition place and seminal cervix outflow. Semen was deposited as deep as possible without distress or trauma cervix mucosa. All Assaf ewes presented cyclic activity before progestagen + eCG treatments (2^nd fortnight of May). Short-term progestagen + eCG treatments were equally efficient (100.0%). About 76.5% of Assaf ewes were pregnant 41 days after AI. Fertility rate was influenced by external Os type, semen deposition place and seminal cervix outflow. However, this rate was not conditioned by vaginal color or lubrication, cervical mucus viscosity, semen preservation technic and semen extender.

Sheep, Assaf, Estrus Synchronization, Artificial Insemination

Fornazari, R. , Mateus, Ó. , Correia, T. , Quintas, H. , Maurício, R. , Conradi, A. , Francisco, L. , Álvaro, A. and Valentim, R. (2018) Estrus Synchronization and Artificial Insemination with Fresh and Chilled Semen in Assaf Ewes. Agricultural Sciences, 9, 8-22. doi: 10.4236/as.2018.91002.