Author(s)

Christophe Massard^1*, Clémence Dubois², Vincent Raspal¹, Pierre Daumar², Yves Sibaud¹, Emmanuelle Mounetou², Mahchid Bamdad², Oscar Komla Awitor¹

¹Université Clermont Auvergne, Institut Universitaire de Technologie, UMR 6533 CNRS/IN2P3, LPC+, Equipe Physico Chimie des Surfaces Nanostructurées, Clermont-Ferrand, France.
²Université Clermont Auvergne, Institut Universitaire de Technologie, UMR INSERM 1240 Imagerie Moléculaire et Stratégies Théranostiques, Groupe “Résistance”, Clermont-Ferrand, France.

The Triple Negative “Basal-like” breast cancer (TNBL) tumours have a high proliferative capacity and develop a resistance phenotype associated with metastases. However, the management of TNBL carcinomas is still not standardized. Among the promising trails, gold nanoparticles could be a relevant tool for the development of a targeted treatment for this breast cancer subtype in monotherapy, associated and/or conjugated with other drugs. In this work, we report the cytotoxicity impact of gold nanoparticles wrapped in Poly-Ethylene Glycol (PEG) on the TNBL HCC-1937 breast cancer cell line. PEG-coated gold nanoparticles (PEG-Au NPs) were synthesized by a two-step method using a reduction process followed by a post-functionalization called PEGylation. PEG-Au NPs were characterized using transmission electron microscopy and X-ray diffraction. The gold content of the samples was determined using atomic absorption spectrometer. The cytotoxicity tests were performed using Sulforhodamine B survival test and resazurin viability test. PEG-Au NPs impact analysis on HCC1937 TNBL cell line showed a clear toxic action of type dose dependent and at long term. These PEGylated gold nanoparticles present a promising tool for the development of tumor-specific radiosensitizing vectors, with or without the association of other treatment strategies.

Breast Cancer, “Basal-Like” Triple Negative, HCC-1937, Gold Nanoparticles, Cytotoxicity

Massard, C. , Dubois, C. , Raspal, V. , Daumar, P. , Sibaud, Y. , Mounetou, E. , Bamdad, M. and Awitor, O. (2018) Cytotoxicity Study of Gold Nanoparticles on the Basal-Like Triple-Negative HCC-1937 Breast Cancer Cell Line. Journal of Biomaterials and Nanobiotechnology, 9, 13-25. doi: 10.4236/jbnb.2018.91002.